基于频域OCT的生物组织折射率测量研究

Refractive index measurement of bio-tissue based on Fourier domain optical coherence tomography

提出了一种利用频域光学相干层析系统测量生物组织折射率的方法。与时域OCT下的光程匹配法相比,该方法充分地发挥了频域OCT测量速度快的优势,只需要在放置样品前后进行两次干涉光谱测量,经傅立叶变换等数据处理后即可得到折射率。测量过程简单,操作简便,测量速度快,极大地缩短了样品在空气中暴露的时间,提高了测量的准确性。利用该方法测量了标准样品熔石英及真实样品黄瓜浅表层1300nm波长下的折射率,验证了该方法的有效性和可靠性。

The measurement of bio-tissue refractive index based on Fourier domain optical coherence tomography(FDOCT)is presented.Compared with optical path matching method based on time domain optical coherence tomography(TDOCT),this method has the advantage of high speed.The refractive index can be deduced by completing Fourier transformation of interference spectra that are taken with and without sample respectively.This method can make the measurement fast and easy to control,and shorten the measurement time to ensure that the measured values are close to physiological status.Through measuring the refractive index of a fused silica and a sub-surface of a cucumber at the wavelength of 1 300 nm,the validity of this method is verified.