ICU环境致病菌对常用消毒剂抗性的研究

Study on disinfectant resistance of pathogens isolated from ICU to common disinfectants

目的研究ICU环境中分离出的致病菌株对常用消毒剂的敏感性,为科学选择和合理使用消毒剂,提高ICU预防性消毒工作质量提供依据。方法通过最小抑菌浓度试验(MIC),检测环境分离菌株对邻苯二甲醛、含氯消毒剂、碘伏和酒精的敏感性,与标准菌株相比较,判断菌株的消毒剂抗性水平。结果邻苯二甲醛对各试验菌的MIC值除大肠埃希菌与标准菌株一致以外,其他试验菌的MIC值均高于标准菌株;碘伏消毒液对90.0%屎肠球菌的MIC值高于标准菌株;含氯消毒液对革兰氏阴性菌的MIC值显著高于标准菌株;酒精对所有实验菌株的MIC值均与标准菌株一致。结论 ICU环境分离出的菌株已经对临床常用的邻苯二甲醛、含氯消毒液和碘伏消毒液产生不同程度的抗性。

Objective To investigate the levels of disinfectant resistance of pathogens isolated from ICU,provide reference for the scientific use of chemical disinfectants and improving the quality of preventive disinfection.Methods Minimum inhibitory concentration(MIC)method was used to detect the sensitivity of environmental isolates to phthalaldehyde,chlorine-containing disinfectant,iodide and alcohol,we compared with standard strains to determine the level of disinfectant resistance of isolated strains.Results The MIC value of phthalaldehyde to each test bacterium was higher than that of the standard strain except for Escherichia coli.The MIC value of iodophor disinfectant for 90.0%enterococcus faecium was higher than that of standard strain,and that of chlorine disinfectant for Gram-negative bacteria was significantly higher than that of standard strain.MIC values of alcohol for all experimental strains were consistent with those of standard strains.Conclusion ICU environmental isolates have developed varying degrees of resistance to clinical commonly used phthalaldehyde,chlorine-containing disinfectant and iodide disinfectant.