白塞病患者及正常人外周血免疫细胞群的比较研究

Contrast study of peripheral blood immune cells in Behcet's disease patients and nomal persons

目的研究白塞病(Behcet’s disease,BD)患者体内细胞免疫水平,我们采用流式细胞术检测了活动期BD患者外周血免疫细胞群的比例变化。方法采用流式细胞术检测了BD患者(n=22),其中活动期BD(active BD,ABD)患者(n=14),BD稳定期(stable BD,SBD)患者(n=8)以及健康体检者(n=22)外周血CD4+T细胞及CD8+T细胞比例与CD28共表达水平与疾病的关系。结果患者体内CD4+T细胞比例与正常人相比并无明显变化,而CD8+T细胞的比例却显著升高(P<0.05),导致CD4+/CD8+的比例明显下降(P<0.05)。此外,BD患者外周血CD28细胞比例与正常人相比无明显变化。进一步分析CD4+T细胞及CD8+T细胞上CD28的表达水平,发现BD患者CD4+T细胞及CD8+T细胞上CD28表达水平较正常人明显降低(P<0.05)。结论结果支持BD患者体内存在免疫细胞群比例的紊乱,值得进一步研究免疫功能缺陷与疾病的关系,为临床诊断白塞病提供了相应的理论依据。

Objective To study cell immunity levels in the patients of Behcet's disease.Methods Flow cytometry was used to detect the ratio of CD4+T cells to CD8+T cells,the expression level and their relatiaonship in 3groups,BD(n=22,ABD,n=14;SBD,n=8),nomal persons(n=22).Results No obvious change of CD4+T ratio was observed between patients and nomal persons,but CD8+T cells increased in patients(P<0.05).Leading to decrease of CD4+/CD8+ratio(P<0.05).No obvious change of CD28 ratio was noted to the nomal persons,but there was obvious decrease of CD28 expression level in CD4+T cells and CD8+T cells among the patients(P<0.05).Conclusion Disorder of immune cells in BD patients is proved,which may be significant in the diagnosis of BD.