摘要
目的检测白介素-6(interleukin-6,IL-6)、胰岛素样生长因子1(insulin-like growth factor-1,IGF-1)在非小细胞肺癌组织和癌旁组织的表达,分析IL-6与IGF-1表达的相关性及其与临床病理特征的关系。方法随机选取150例非小细胞肺癌和100例癌旁良性组织标本,采用免疫组织化学检测组织中IL-6、IGF-1的表达及两者表达的相关性,并分析其表达与临床病理的关系。结果肺癌组IL-6、IGF-1阳性表达率84.00%、80.00%分别显著高于癌旁正常组织阳性表达率23.00%和28.00%。IL-6肿瘤组织阳性表达率在吸烟指数≥400的患者为90.91%,显著高于吸烟指数<400者,IL-6在腺癌阳性表达率93.24%高于鳞癌77.63%。IGF-1肿瘤组织阳性表达率在低分化患者中显著高于中、高分化患者,III、IV期患者肿瘤组织IGF-1表达率显著高于I、II期患者。非小细胞肺癌组织中,IL-6阳性表达与IGF-1阳性表达之间呈正相关。结论 IL-6、IGF-1蛋白在非小细胞肺癌组织中明显高表达,IL-6高表达与更高的吸烟水平和腺癌组织类型相关,IGF-1高表达与更低的肿瘤分化程度和更晚的TNM分期有关,更重要的是,IL-6阳性表达与IGF-1阳性表达正相关,IL-6和IGF-1通路可能存在交叉作用,两者联合检测或抑制是潜在的诊断和治疗方向。
Objective To investigate the expressions of IL-6 and IGF-1 in NSCLC and para-carcinoma tissues,and to elucidate the correlation between their expressions and their association with clinical factors. Methods IL-6 and IGF-1 were detected by immunohistochemical analysis in 150 NSCLC and 100 para-carcinoma samples. The correlation of IL-6 and IGF-1 expressions and the association between IL-6,IGF-1 and clinical factors were analyzed. Results Overexpressions of IL-6 and IGF-1 were detected by immunohistochemical analysis in 84. 00% and 77. 00% of the NSCLC samples,compared to 77. 00% and 72. 00% in para-carcinoma tissue. High expression of IL-6 related with higher smoking index,and high expression of IGF-1 associated with higher TNM-stages and poorer differentiation. The expression of IL-6 was positively correlated with that of IGF-1. Conclusion There are overexpressions of IL-6 and IGF-1in NSCLC tissues. Overexpression of IL-6 relates with higher smoking index,and overexpression of IGF-1 relates with higher TNM-stages and poorer differentiation. More importantly,potential crosstalk may exist between IL-6 and IGF-1 signaling pathways. Co-targeting IL-6and IGF-1 signaling may be a more effective way in diagnosis and therapy for NSCLC.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2015年第5期399-403,共5页
Chinese Journal of Histochemistry and Cytochemistry
基金
湖北省自然科学基金资助(2014CKB503)
