温热抑制人胃癌SGC7901细胞增殖、迁移

Inhibition of proliferation and migration by hyperthermia in the human gastric cancer cell line SGC7901

目的探讨温热对人胃癌SGC790l细胞增殖、迁移的影响。方法对照组常温(37℃)下培养人胃癌SGC790l细胞,实验组43℃水浴加热0.5h、1h、2h、3h后培养24h,采用倒置显微镜观察胃癌细胞的形态结构变化;Hoechst-33258荧光染色观察细胞核的变化;四甲基偶氮唑盐比色法(MTT)检测细胞增殖抑制;细胞划痕愈合实验观察温热对胃癌细胞的运动迁移能力的影响;体外细胞侵袭实验(Transwell实验)观察温热对胃癌细胞侵袭能力的影响。结果温热后细胞明显皱缩、变圆及细胞漂浮,3h大部分细胞漂浮;荧光染色显示温热后部分细胞核内出现浓染致密的颗粒块状荧光,胞核固缩、染色质高度凝聚和碎裂;MTT实验提示温热可明显抑制SGC790l细胞生长;细胞划痕实验发现SGC790l细胞温热1h、2 h后细胞迁移距离均明显小于对照组,温热3h后细胞基本未发生迁移;Transwell实验提示SGC790l细胞温热后细胞侵袭能力明显下降。结论温热对胃癌SGC790l细胞具有明显的杀伤作用,温热可明显抑制胃癌SGC790l细胞增殖和侵袭迁移能力。

Objective To investigate the effect of hyperthermia on proliferation and migration of the human gastric cancer cell line SGC7901. Methods Human gastric cancer MKN45 cells were resuscitated and cultured in vitro. Following thermotherapy at 43 ℃for 0,0. 5,1,2 or 3h,human gastric cancer SGC7901 cells were further cultured for 24 h. Their morphology was observed by inverted microscopy. Nuclear analysis was evaluated by Hoechst-33258 fluorescent staining. Cell proliferation was determined by MTT. Cell migration and cell invasion were evaluated by wound healing assay and transwell assay. Results Inverted microscopy revealed shrinked,rounded and floated SGC7901 cells,and Hoechst-33258 fluorescent staining demonstrated that there were nuclear condensation and fragmentation after thermotherapy. MTT assay showed that the growth of SGC7901 cells was inhibited. Cell migration was obviously reduced compared to that of the controls. No obvious migration was detected at 3 h of treatment. Invasion of SGC790 l cells was reduced significantly. Conclusions Thermotherapy has a destructive effect on GC7901 cells,and significantly inhibits their proliferation,migration and invasion.