肝细胞癌中API-5水平升高与细胞凋亡减少

Increase of API-5 level and decrease of cell apoptosis in hepatocellular Carcinoma

目的为了为揭示肝癌发生的分子机制提供线索,了解肝细胞癌中API-5表达与细胞凋亡之间的关系。方法手术取得并确诊肝细胞癌和癌旁组织各16例,通过免疫组织化学二步法检测凋亡抑制因子-5(apoptosis inhibitor-5,API-5)的表达,利用TUNEL法检测其细胞凋亡,API-5表达水平和细胞凋亡水平用IPP v5.1图像分析软件进行检测分析。结果在肝细胞癌组肝细胞中,API-5蛋白水平明显高于癌旁组织组,而细胞凋亡水平则明显低于癌旁组织组。结论肝细胞中高水平的API-5作为细胞凋亡抑制蛋白,抑制了细胞的凋亡,打破了其细胞增殖和凋亡之间的平衡,导致肝细胞癌发生。

Objective To provide a clue to reveal the molecular mechanism of liver carcinogenesis;to understand the relationship between API-5 expression and cell apoptosis in hepatocellular carcinoma(HCC).Methods HCC and carcinoma-adjacent tissues of 16 cases were obtained after surgery and diagnosis were confirmed by HE staining.API-5 protein expression of these tissues was detected by the two-step immunohistochemical method,and their apoptosis detected by TUNEL.IPP-v-5.1 image analysis software was used to detect and analyze API-5 expression and cell apoptosis.Results The expression level of API-5 protein in the HCC group was remarkably higher than that in the carcinoma-adjacent tissue group.The level of apoptosis in HCC was remarkably lower than that in carcinoma-adjacent tissue.Conclusion High expression of API-5,an apoptosis inhibiting protein,in liver cells may inhibit cell apoptosis,break the balance between cell proliferation and apoptosis,and thus induce hepatocellular carcinogenesis.