白血病和正常血细胞的一些细胞因子及受体表达的比较研究

Expression of cytokines and their receptors in leukemia cell lines and normal blood cells

目的 :研究白血病细胞和正常血细胞的细胞因子及相应受体 (R)的表达及其意义。方法 :采用逆转录 -聚合酶链反应方法检测红白血病细胞系 (HEL、K562 )、髓系白血病细胞系 (HL - 60 )、单核细胞白血病细胞系(U937)、巨核细胞白血病细胞系 (DAMI、MEG - 0 1 )、T淋巴瘤细胞系 (HUT78)及EB病毒感染的B细胞系 (CA)和正常血细胞 (CD34+ 造血干细胞、外周血单个核细胞、成熟粒细胞、巨核细胞及血小板 )的多种细胞因子及受体的表达。结果 :①CD34+ 造血干细胞同时表达IL - 1 (α、β)、IL - 3、IL - 6、GM -CSF、G -CSF及相应受体和干细胞因子受体(SCFR)、血小板生成素受体 (MPL)基因 ,在成熟粒细胞仅表达IL - 6、IL - 6R、G -CSFR、GM -CSF ,巨核细胞、血小板仅表达IL - 3R、IL - 6、IL - 6R、MPL。而TGFβ1 、TNFα及相应受体在上述正常血细胞均有持续表达。②HEL、K562、HL -60、U937、DAMI、MEG - 0 1、HUT78及CA可同时表达至少两种以上正调控因子及相应受体 ,而负调控因子TGFβ1 、TNFα及受体在上述各细胞系均有表达。结论 :白血病细胞株细胞存在正性多自泌环节 ,白血病细胞的正。

AIM: To study the expression of cytokines and their receptors in leukemia cell lines and normal blood cells. METHODS: RT-PCR was used to detect expression of mRNA for cytokines in leukemia cell lines(HL-60,U937,K562,HEL,DAMI,MEG-01,HUT78 and CA) and normal blood cells, including CD34 + cells, megakaryocytes,platelets, peripheral mononucleates cells and granulocytes. RESULTS: ①CD34 + cells simultaneously expressed mRNA for IL-1(α,β),IL-3, IL-6 , G-CSF, GM-CSF and their receptors and SCFR,MPL as well. The granulocytes only expressed IL-6,IL-6R,G-CSFR,GM-CSF. Megakaryocytes and platelets only expressed IL-3R,IL-6,IL-6R,MPL.Interestingly, TGFβ 1 ,TNFα and their receptors sustained to express in normal cells.②Most leukemia cell lines were found to simultaneously express at least two or more stimulating cytokines and receptors ,while TGFβ 1 , TNFα and their receptors were expressed in all the leukemia cell lines we observed. CONCLUSIONS: ①Multi-autocrine loops exist in leukemia cells;②Imbalance of autocrine loops of positive and negative cytokines may be related to leukemia.