五味子乙素对氧化损伤的晶状体上皮细胞凋亡的影响

Effects of schisandrin B on apoptosis of lens epithelial cells treated with H_2O_2

目的 :探讨五味子乙素 (SchB)对实验性氧化损伤大鼠晶状体上皮细胞 (LEC)凋亡的影响。方法 :采用无菌操作摘取SD大鼠双眼 ,并在手术显微镜下分离晶状体 ,随机分为空白对照组、过氧化氢组 (H2 O2 )、吡诺克辛(PS)组和SchB组。使晶状体孵育在 30 0 μmol·L- 1 H2 O2 培养液中复制LEC凋亡模型 ,同时加入终浓度为 0 5mmol·L- 1 的SchB ,置二氧化碳培养箱共同孵育 2 4h。取晶状体前囊膜 ,采用TUNEL法检测LEC凋亡及凋亡率 ,透射电子显微镜观察LEC超微结构改变和凋亡小体形成。结果 :H2 O2 组LEC凋亡率 (92 0± 2 6)显著高于空白对照组(3 5± 1 8) (P <0 0 1 ) ;SchB组LEC凋亡率 (1 3 8± 3 0 )显著低于H2 O2 组 ,且与PS组 (56 0± 9 9)有显著的差异 (P<0 0 5)。超微结构研究显示 ,H2 O2 组绝大多数LEC发生凋亡 ,并呈凋亡各期严重改变的全过程 ;SchB组仅少数LEC发生凋亡 ,并多为早期或中期的轻微改变。结论 :SchB可明显抑制实验性氧化损伤大鼠LEC凋亡 ,且明显优于PS滴眼液。

AIM: To investigate the effects of Schisandrin B (Sch B) on apoptosis of lens epithelial cells (LEC) treated with H 2O 2. METHODS: Eyes in SD rats were excised and lenses were separated under operating microscope in sterilized condition. Lenses were divided randomly into four groups with different treatment: control group, hydrogen peroxide group (H 2O 2), pirenoxine sodium group (PS) and schisandrin B group (Sch B). Lenses were incubated in CO 2 incubator for 24 h with 300 μmol·L -1 H 2O 2 and with or without 0 5 mmol·L -1 Sch B. LEC aoptosis and apoptosis rate were measured by TUNEL method. Ultrastructure changes and apoptosis bodies of LEC were observed via transmitted electron microscope. RESULTS: (1) Apoptosis rate in H 2O 2 group (92.0±2.6) was significantly higher than that in control group (3.5±1.8). Apoptosis rate in Sch B group (13.8±3.27) was remarkably lower than that in H 2O 2 group and PS group. (2) Ultrastructure observation indicated that apoptosis cells occurred in most LEC in H 2O 2 group and the changes were severe presenting different stages. While a few apoptosis cells were observed in Sch B group, the changes were slight and most of them were in early and middle stages. CONCLUSION: These data indicated that Sch B significantly inhibited apoptosis of LEC during experimental oxidative injury, the effects were stronger than PS.