摘要
在40例膀胱移行上皮乳头状瘤及移行细胞癌Ⅰ、Ⅱ、Ⅲ级病变的石蜡组织切片上,采用AgNOR染色技术,进行了核仁组成区嗜银蛋白的研究。结果表明,除移行上皮乳头状瘤与移行细胞癌Ⅰ级间P>0.05,AgNOR计数无显著差异外,在Ⅰ、Ⅱ、Ⅲ三级移行细胞癌中AgNOR计数均有显著意义(P<0.01)。当对移行细胞癌进行分级遇到困难时,可能采用AgNOR技术对肿瘤分级的诊断具有辅助作用。
Using a silver staining technique, nucleolar organizer region-associated proteins (AgNOR) have been studied in paraffin section of 4 bladder papillomas, 15 Grade I transitional carcinomas, 13 Grade Ⅱ and 8 Grade Ⅲ. The sections were examined using a ×100 oil-immersion lens and the AgNOR were enumerated with ease. A highly significant difference were found between the numbers of AgNOR in the nuclei of Grade Ⅰ transitional carcinomas and the Grade Ⅱ, the Crade Ⅱ and the Grade Ⅲ. But a significant difference was not between the bladder papillomas and the Grade Ⅰ transitional carcinomas. It is suggested that the AgNOR technique will find application to histopathology grading in bladder transitional carcinomas.
关键词
核仁组成区
移行细胞癌
膀胱肿瘤
nucleolar organizer regions
bladder transitional carcinomas
histopathologic grading