摘要
以红王子锦带带腋芽的茎段为外植体进行离体培养基的筛选。结果表明 :红王子锦带的离体培养以MS、WPM为基本培养基 ,附加适当比例的细胞分裂素(0.1~1mg·L-16 -BA)和生长素(0.01~0.1mg·L-1NAA)为宜。本试验采用方差分析及多重比较方法对试验数据进行处理 ,筛选出红王子锦带各培养阶段最佳培养基分别为:(1)外植体诱导培养基(A2) :MS +6 -0.1mg·L -1BA +0.01mg·L -1NAA ;(2)分化及继代培养基(B3) :MS +0.5mg·L -16 -BA +0.05mg·L -1NAA ;(3)生根培养基(C2) :WPM +0.4mg·L -1NAA。
The stem-segments with axillary buds of Weigela floridacv. red prince were used as the explants to sceen the suitable media in vitro culture.The results indicated that MS or WPM as basic medium,added suitable proportion cytokinin(0.1-1mg·L-16-BA)and auxin(0.01-0.1mg·L-1NAA)was appropriate.Through variance analysis and multiple comparison,the appropriate media for different culture stages were determined as:(1)for derivation A2:MS+0.1mg·L-16-BA+0.01mg·L-1NAA;(2)for differentiation and subculture B1:MS+0.5mg·L-16-BA+0.05mg·L-1NAA;(3) for rooting C2 :WPM+0.4mg·L-1NAA.
出处
《沈阳农业大学学报》
CAS
CSCD
2002年第3期202-204,共3页
Journal of Shenyang Agricultural University
关键词
基因筛选
工王子锦带
茎段
离体培养基
Weigela florida cv.red prince
stem-segment
in vitro culture
medium