摘要
以 2型鸭疫里氏杆菌江西分离株为菌种 ,制备了含不同菌数的 2型铝胶疫苗 ,分别经颈背部皮下接种 5日龄雏鸭 (0 .5mL/羽 ) ,免疫后 5 ,1 0 ,1 5d分别用同源菌株攻击 ,保护指数为 60左右 ;于 2 5日龄第 2次免疫 ,二免后第 5d的保护指数达 91 .7～ 1 0 0。本试验还对鸭疫里氏杆菌的培养条件、灭活条件进行了优化。
Three inactivated vaccines, respectively contained the equivalent of 1×10 9,2×10 9,3×10 9 colony-forming units of bacteria per 0.5-ml dose, were prepared from the serotype 2 of Pasteurella anatipestifer (RA) in shaken tryptose broth. A single subcutaneous inoculation of vaccine in 5-day-old ducklings gave adequate protection (PI=60) against challenge with the homologous of these vaccines; the second inoculation protected above 90% of 25-day-old ducklings against experiment challenge 5 days after the inoculation. In the experiment, the conditions for RA culture and inactivation were optimized.
出处
《江西农业大学学报》
CAS
CSCD
2002年第5期569-573,共5页
Acta Agriculturae Universitatis Jiangxiensis
关键词
鸭
疫里氏杆菌
灭活疫苗
研制技术
Pasteurella anatipestifer
serotype 2
inactivated vaccine
