金银花类中药提取物清除自由基的作用

Scavenging Capacities on Radicals of Flos Lonicerae by Chemiluminescence

目的 比较金银花类中药以及忍冬不同提取部位清除自由基的作用。方法 生物化学发光法。结果 所检测样品对 3种自由基系统 ( O- 2 、· OH、H2 O2 )都有不同程度的清除作用。同一物种 ,道地产区河南、山东产忍冬清除自由基的作用强于江苏产忍冬 ;不同物种 ,清除自由基能力大小依次为灰毡毛忍冬、忍冬、细毡毛忍冬、黄褐毛忍冬、菰腺忍冬。忍冬不同提取部位清除自由基能力大小依次为正丁醇部位、乙酸乙酯部位、水提液。绿原酸、忍冬苷均具有较强的清除作用 ,而忍冬苷稍强于绿原酸。结论 金银花类中药清除自由基能力随产地、物种不同而有差异 。

AIM To study the scavenging capacities on radicals of Flos Lonicerae . METHOD Chemiluminescence. RESULTS All the samples show the scavenging capacities on three kinds of radicals to some extent. As the same species,the scavenging capacities of the samples from Henan and Shandong are stronger than those from Jiangsu,and as the different species,the scavenging capacities in descending order are L.macranthoides,L.japonica,L.similis,L. fulvotomentosa and L.hypoglauca. With regard to L.japonica ,the scavenging capacities of n-BuOH extract are stronger than those of EtOAc extract. CONCLUSION The scavenging capacities on radicals vary from locality and species,and are highly relative to the contents of phenolic acids.