白花蛇舌草不同提取工艺对抗肿瘤活性的影响

Influence of Extraction Process of Hedyotis diffusa on Anti-tumor Activity

目的 为研制低毒高效的抗癌制剂 ,确定不同的白花蛇舌草提取物 (汤剂、亲脂提取物、粗多糖 )的抗肿瘤作用。方法 采用水提取法、醇提取法、脱脂水提醇沉法分别制备了白花蛇舌草汤剂、亲脂提取物和粗多糖。用 S-180细胞植入小鼠作为模型 ,进行了抑制肿瘤的试验。结果 白花蛇舌草的水提液 (汤剂 )对 S- 180肿瘤细胞没有明显的抑制作用 ;白花蛇舌草水溶性的多糖和亲脂提取物对 S- 180肿瘤细胞具有显著的抑制作用 ;在抑瘤的同时 ,多糖部分具有对化学损伤的保护作用 ;水溶性的多糖和亲脂提取物合并给药并未显示协同抗肿瘤活性。结论 白花蛇舌草的脂溶性部位和多糖具有良好的抗肿瘤应用前景 。

AIM To develop less toxic and more effective anti-tumor drugs with the medicinal herb, the various extracts from the herb Bai-Hua-She-She-Cao( Hedyotis diffusa ) were prepared and determined of their single and multi action of anti-tumor. METHODS Decoction, lipophilic extract and crude polysaccharide were prepared from Hedyotis diffusa , with water extraction, alcohol extraction and defatting/water extraction and alcohol precipitation, respectively. Anti-tumor activities were tested with tumor-model in vivo (Sarcoma-180 cells implanted in mice). RESULTS Experimental results indicated that the elementary aqueous extract of H. diffusa had no marked anti-tumor effect on Sarcoma-180 cells implanted in mice. The water-soluble crude polysaccharides separated from H. diffusa and the lipophilic extract of H. diffusa showed the anti-tumor activities,and showed the protective effect on chemotherapeutic damage. Combined administration of the lipophilic extract and the crude polysaccharides did not show the synergistic anti-tumor activity. CONCLUSION The experimental results indicated that the herb will have prospect of application to anti-tumor; the separated extraction is better than simple decoction for the treatment; the further studies on the multi-use of the herbal constituents will be needed.