当归浸膏片与当归极细粉片和当归超微片的质量比较

Quality comparison of Angelica extract,Angelica superfine powder pills and Angelica superfine

目的比较研究当归3种不同粒径当归超微粉碎片的质量。方法定性检测用薄层色谱法与超声提取,以环己烷-二氯甲烷-乙酸乙酯-甲酸(4∶1∶1∶0. 1)为展开剂,阿魏酸为对照,紫外365 nm波长处检测。定量检测用高效液相色谱(HPLC)法,梯度洗脱。色谱条件:色谱柱:Kromasil C18色谱柱(4. 6 mm×250. 0 mm,5. 0μm),流速:1. 0 mL·min^(-1),检测波长:316 nm,柱温:28℃,流动相:乙腈-0. 05%磷酸水(16∶84)。结果当归超微粉片、极细粉片、浸膏片与对照品薄层色谱在相应的位置上显相同颜色的荧光斑点。阿魏酸含量:当归超微粉碎片19. 60μg·mL^(-1),当归极细粉片19. 57μg·mL^(-1),当归浸膏片16. 35μg·mL^(-1)。结论不同粉末粒径会影响当归中阿魏酸的溶出;用HPLC法测定当归中阿魏酸的含量,操作简单、可靠。

Objective To study the quality of Angelica ultrafine comminuted tablets of three different particle sizes. Methods After ultrasonic extraction,qualitative detection was performed by thin-layer chromatography with ultrasonic. Cyclohexane-dichloromethane-ethyl acetate-formic acid( 4∶ 1∶ 1∶ 0. 1) used as a developing agent and ferulic acid as a control. Ultraviolet wavelength was 365 nm. The high performance liquid chromatography( HPLC) was used for quantitative detection. Chromatographic conditions: Column: Kromasil C18 column( 4. 6 mm × 250. 0 mm,5. 0 μm),flow rate: 1. 0 mL · min-1,detection wavelength: 316 nm,co-lumn temperature: 28 ℃,mobile phase: acetonitrile-0. 05%phosphoric acid water( 16∶ 84) with gradient elution. Results Angelica microcrush tablets,extremely fine powder tablets,extract tablets and reference materials TLC showed the same color fluorescent spots at corresponding positions. Ferulic acid content: Angelica ultrafine comminution tablets was 19. 60 μg · mL-1,Angelica extremely fine powder tablets was 19. 57 μg·mL-1,Angelica extract tablets was 16. 35 μg·mL-1.Conclusion The particle size of different powders will affect the dissolution of ferulic acid in Angelica sinensis. Meanwhile,the HPLC method for the determination of ferulic acid in Angelica is simple and reliable.