川芎嗪对小儿急性髓性白血病KG-1a细胞增殖及表面标志物表达的影响

Effect of ligustrazine on the cell proliferation and the expression of surface marker of pediatric acute myeloid leukemia KG-1a cells

目的研究川芎嗪对小儿急性髓性白血病KG-1a细胞增殖及表面标志物表达的影响。方法取对数期KG-1a细胞,低,中,高剂量实验组分别以5,50,100μg·mL^(-1)川芎嗪溶液处理,对照组细胞则以等量生理盐水处理,分别继续培养24,48,72 h。分别于光学显微镜下观察各组细胞干预48 h后细胞形态学变化;以噻唑蓝(MTT)检测各组细胞培养24,48,72 h后增殖抑制情况;以流式细胞术检测高剂量实验组KG-1a细胞表面抗原CD7及CD56表达情况。结果低、中、高剂量实验组KG-1a细胞24 h增殖抑制率分别为(20. 01±0. 08)%,(32. 43±0. 09)%,(47. 38±0. 09)%; 48 h增殖抑制率分别为(35. 94±0. 09)%,(50. 82±0. 10)%,(60. 06±0. 12)%; 72 h增殖抑制率分别为(40. 26±0. 10)%,(62. 34±0. 12)%,(68. 85±0. 14)%;实验组KG-1a细胞增殖抑制率随川芎嗪作用浓度的增加及作用时间的延长逐渐升高,差异均有统计学意义(均P <0. 01)。给药48 h后,对照组与高剂量实验组KG-1a细胞CD7表达率分别为(94. 53±3. 67)%,(79. 86±5. 02)%; CD56表达率分别为(92. 93±4. 11)%,(89. 41±3. 89)%,差异均有统计学意义(均P <0. 01)。结论川芎嗪可显著抑制小儿急性髓性白血病KG-1a细胞增殖,并可有效抑制其表面抗原CD7及CD56表达。

Objective To explore the effect of ligustrazine on the cell proliferation and the expression of surface marker of pediatric acute myeloid leukemia KG-1a cell.Methods The logarithmic phase KG-1a cell in the experimental-L/-M/-H groups were managed by 5,50,100μg·mL-1 ligustrazine solution respectively,and the KG-1a cell in the control group were managed by equivalent normal saline,all groups continuely managed for 24,48,72 h respectively.The changes of cell morphology 48 h after managed were observed under optical microscope;the proliferation inhibition of KG-1a cell in each group 24,48,72 h after managed were detected by methylthiazolyldiphenyl-tetrazolium(MTT);the expression of KG-1a cell surface antigen CD7 and CD56 in the experimental-H group were detected by flow cytometry.Results At 24 h after treatment,the proliferation inhibition rates of the experimental-L/-M/-H groups were(20.01±0.08)%,(32.43±0.09)%,(47.38±0.09)%,respectively;at 48 h after treatment,those were(35.94±0.09)%,(50.82±0.10)%,(60.06±0.12)%,respectively;at 72 h after treatment,those were(40.26±0.10)%,(62.34±0.12)%,(68.85±0.14)%respectively.The proliferation inhibition rates of KG-1a cell were increased in a dose-dependent manner(P<0.01).At 48 h after treatment,the CD7 expression rates of KG-1a cell in control group and experimental-H group were(94.53±3.67)%,(79.86±5.02)%,respectively;the CD7 expression rates were(92.93±4.11)%,(89.41±3.89)%,respectively;There were significant differences between the two groups(P<0.01).Conclusion Ligustrazine can effectively inhibit the pediatric acute myeloid leukemia KG-1a cell proliferation and the expression of surface antigen CD7 and CD56.