乳腺癌组织中乳腺癌扩增序列2与β-连环蛋白的表达及其相关性研究

Expressions and correlation of breast cancer amplified sequence 2 and β-catenin in breast cancer

目的探讨乳腺癌组织中乳腺癌扩增序列2(BCAS2)和β-连环蛋白(β-catenin)的表达及其临床意义。方法用免疫组织化学法检测乳腺癌组织芯片中BCAS2和β-catenin的蛋白表达,并分析两者表达的相关性以及两者的表达率与乳腺癌患者临床病理特征的关系。用双荧光素酶报告基因实验验证BCAS2对β-catenin的调控作用。结果乳腺癌组织和癌旁组织中BCAS2的阳性表达率为75.7%和37.5%,β-catenin的阳性表达率为68.1%和27.1%,差异均有统计学意义(均P<0.001),且二者表达呈正相关(r=0.584,P<0.001)。BCAS2和β-catenin蛋白在淋巴结转移及临床分期为Ⅲ~Ⅳ级的患者中表达率明显增高,敲低BCAS2可显著抑制β-catenin的荧光素酶活性。结论 BCAS2及β-catenin在乳腺癌的发生发展中有作用,且二者表达呈正相关。

Objective To investigate the expressions of breast cancer amplified sequence 2(BCAS2)andβ-catenin in breast cancer and to analyze its clinical significance.Methods Immunohistochemical method was used to detect the expressions of BCAS2andβ-catenin in breast cancer tissues and tumor-adjacent normal breast tissues.Statistical analysis was carried out to analyze the relationship between BCAS2andβ-catenin expression,as well as the relationship between these expression and clinicopathologic characteristics;dual-luciferase reporter gene assay was used to verify regulation of BCAS2onβ-catenin.Results The positive expression rates of BCAS2 in breast cancer tissues and adjacent tissues were 75.7%and 37.5%,and the positive rates ofβ-catenin were 68.1%and 27.1%,the differences were statistically significant(both P<0.001),and the expression of them was positively correlated(r=0.584,P<0.001).The expression of BCAS2andβ-catenin protein was closely related to lymph node metastasis and histological grade(Ⅲ-Ⅳ).Knockdown BCAS2can significantly inhibit luciferase activity ofβ-catenin.Conclusion The BCAS2andβ-catenin may play a critical role in the occurrence and development of breast cancer,their expressions are positively correlated.