桔梗五倍子提取液的抑菌实验研究

Experimental Study on Bacteriostatic Effects of Platycodon Grandiflorum and Chinese Gall Extract

目的观察中草药五倍子、桔梗及桔梗五倍子提取液的抑菌作用。方法中药五倍子、桔梗采用0.9%生理盐水恒温金属浴中提取有效成分,制成冻干粉,按桔梗:五倍子2:1比例混合制成提取液并制成冻干粉。参照《全国临床检验操作规程》关于最低抑菌浓度(MIC)的检测方法,检测五倍子、桔梗及桔梗五倍子对大肠埃希菌、金黄色葡萄球菌的杀菌抑菌作用,同时与生理盐水(NS)和壬苯醇醚(NP-9)对照组进行对比研究。结果桔梗对金黄色葡萄球菌及大肠埃希菌均无明显的抑菌作用,五倍子及桔梗五倍子混合液对金黄色葡萄球菌有明显的抑菌作用,对大肠埃希菌无明显作用,使用浓度范围内NP-9无抑菌作用。结论中草药桔梗五倍子提取液具有体外抑菌效果,可作为体外抑菌的中草药制剂。

Objective To study the antibacterial effects of Chinese gall,platycodon grandiflorum or the compound of both. Methods To extract effective components of Chinese gall and platycodon grandiflorum( 0. 9% saline thermostat metal bath). According to 2: 1 proportion of platycodon grandiflorum: Chinese gall,we made them into freeze-dried powder. Refering to the minimal inhibitory concentration( MIC) of Chinese gall,platycodon grandiflorum or the compound of both,we study the antibacterial effects on Escherichia coli,staphylococcus aureus,while were compared and analyzed with NS or NP-9. Results Platycodon grandiflorum showed no effect on the antibacterial effects tested.Chinese gall and the compound of both had the effects on the bacteriostatic action of staphylococcus aureus,while had no antibacterial effects of escherichia coli. Conclusion The compound of Chinese gall and Platycodon grandiflorum had the antibacterial effects,and can be used as in vitro antimicrobial agents.