黄芩苷对高同型半胱氨酸血症ApoE基因缺陷小鼠动脉粥样硬化发生中炎性因子表达的影响

Effects of Baicalin on Expression of Inflammatory Factors in Development of Atherosclerosis in ApoE-/-mice with Hyperhomocysteinemia

目的研究黄芩苷对高同型半胱氨酸血症(Hyperhomocysteinemia,HHcy)Apo E基因缺陷小鼠动脉粥样硬化发生中炎性因子表达的影响,探究黄芩苷抗动脉粥样硬化的机制。方法将30只雄性Apo E基因缺陷小鼠,随机分配到普通组(A组)、高同型半胱氨酸血症组(B组)、黄芩苷治疗组(C组)。A组给予普通饲料喂养;其他两组在普通饲料喂养的基础上添加2%L-蛋氨酸,喂养8周,于第4周A组和B组给予每天1次生理盐水0.4 m L灌胃,C组给予黄芩苷配置液150 mg/kg/d(稀释为0.4 m L)灌胃。实验结束后处死动物采血、取材。采用高效液相色谱法检测血清Hcy浓度;采用酶联免疫吸附法(ELISA)检测血浆hs-CPR、TNF-α、MCP-1水平;HE染色观察形态学变化,免疫组织化学检测主动脉组织MCP-1蛋白的表达水平,采用Leica Qwin V3图像分析软件。结果 8周后B、C组血清Hcy、hs-CPR、TNF-α、MCP-1水平、斑块面积及主动脉组织MCP-1蛋白的表达水平较A组明显升高(P<0.01);C组血清Hcy、hs-CPR、TNF-α、MCP-1水平、斑块面积及免疫组织化学MCP-1表达水平显著低于B组(P<0.01或P<0.05)。结论高同型半光氨酸血症可促进动脉粥样硬化的形成,黄芩苷能通过降低血浆及动脉组织中炎性因子表达的水平,对动脉粥样硬化的形成、进展进行干预。

Objective To observe the effects of baicalin on the expression of inflammatory factors in the development of atherosclerosis in apo E-/- mice with hyperhomocysteinemia,in order to ivestigate the possible mechanisms of baicalin intervention on atherosclerosis. Methods30 male apo E-/- mices were randomly divided into 3 groups: normal control group( group A),HHcy group( group B) and baicalin dose group( group C). Group A was fed with normal diet,simultaneously the rest groups with the same diet based on the addition of 2% Lmethionine / d / fed,feeding 8 weeks. In the fourth of the experimental period group A and group B were given normal saline 2ml 1 ig per day,at the same time group C were given baicalin 150 mg / kg / d( dilution of 0. 4ml),day 1. At the end of the experiment,animals were killed,serum Hcy concentration was detected by high efficiency liquid chromatography,enzyme-linked immunosorbent assay( ELISA) were performed to examine the protein expression levers of hs-CPR,MCP-1 and TNF-α; HE staining of aorta were used to observer the histomorphological changes and measure the size of aortic plaques,aortic MPC-1 expression was detected by immunohistochemistry,the Leica Qwin V3 image analysis software was adopted. Results Serum Hcy,hs-CPR,TNF-α,MCP-1 in plasma,the aortic plaques area and aortic MPC-1 expression of group B and C increased obviously compared with group A( P < 0. 01); Hcy,hs-CPR,TNF-α,MCP-1 in plasma,the aortic plaques area and aortic MPC-1 expression of group C was lower than that of group B obviously( P < 0. 01 or P < 0. 05). Conclusions Hyperhomocysteinemia could promote the progression of atherosclerosis. Baicalin could intervene the progress of atherosclerosis development by lowering inflammatory factors levels.