HPLC法测定七子二仙胶囊中淫羊藿苷含量

Determination of Icariin in Qizierxian capsule by High performance Liquid Chromatography

目的 建立反相高效液相色谱法测定七子二仙胶囊中淫羊藿苷含量的方法。方法 样品经 70 %乙醇超声提取 ,SpherisorbC18色谱柱 ,0 .0 2mol·L-1磷酸盐 (用盐酸调节pH值至 3.0± 0 .1) -甲醇 (5 9∶4 0 )为流动相 ;检测波长为 2 70nm。结果 淫羊藿苷的线性范围为 1～ 30 0 μg·ml-1,r =0 .9996 ,最低检测浓度 0 .2 μg·ml-1,平均加样回收率 96 .6 % (n =5 )。结论 方法可靠 ,可用于七子二仙胶囊质量控制。

Aim To establish a HPLC method for determination icarrin in Qizierxian capsule.Methods Icarrin were extracted by 70% ethanol using ultrasonic and determined by HPLC,using Spherisorb C 18 (4.6×200mm) column,0.02mol·L -1 phosphate buffer solution(pH 3.0±0.1) methanol(59∶40) as the mobile phase. The detection wavelength was 270nm.Results The linearity range was 1～300μg·ml -1 ,r=0.999 6,minimal determinable content was 0.2μg·ml -1 and average recovery was 96.6% (n=5).Conclusion The method can be used to control the quality of preparation.