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Construction of transgenic vector of mouse dentin sialoprotein 被引量：2

Construction of transgenic vector of mouse dentin sialoprotein
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摘要 Objective To construct transgenic vectors of mouse DSP.Methods To construct pcDNA3.1 CX by substituting CMV promoter of pcDNA3.1 with promoter cβ actin,and establish the ultimate transgenic vector by cloning DSP coding sequence into pcDNA3.1 CX.Result s Enzyme digestion and sequencing are consistent with expected.Conclusion The transgenic vector of mouse DSP was constructed successfully. Objective To construct transgenic vectors of mouse DSP.Methods To construct pcDNA3.1 CX by substituting CMV promoter of pcDNA3.1 with promoter cβ actin,and establish the ultimate transgenic vector by cloning DSP coding sequence into pcDNA3.1 CX.Result s Enzyme digestion and sequencing are consistent with expected.Conclusion The transgenic vector of mouse DSP was constructed successfully.
作者 孙汉堂 肖明振 吴补领 费俭
机构地区 College of Stomatology Shanghai Institute of Biochemistry and Cell Biology Collegeof Stomatology
出处 《中国临床康复》 CSCD 2002年第23期3608-3608,3610,共2页 Chinese Journal of Clinical Rehabilitation
关键词 小鼠 牙本质涎蛋白 转基因载体 PCR mouse dentin sialoprotein transgenic vector
分类号 R78 [医药卫生—口腔医学] Q78 [生物学—分子生物学]
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参考文献7

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同被引文献14

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引证文献2

中国临床康复
2002年 第23期

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