垃圾堆肥高效纤维素分解菌的筛选与培育技术

Study on preparation technology of cellulolytic organisms for composting

从堆肥、马粪、果园土、污泥等 2 0个样品中 ,分离筛选出 3株对滤纸分解旺盛的纤维素分解菌 :C1 、C2 、C3,并外购康氏木霉、白腐菌、变色栓菌一起作为出发株 ,经紫外诱变处理后 ,在含葡萄糖的产酶培养基平板上筛选到能形成较大透明圈的突变株 ,并进行 CMC酶活、微晶纤维素酶活及天然粗纤维分解能力测定。实验结果证明白腐菌经紫外线照射 60 s诱变而得的 C1 6 不仅透明圈大 ,CMC酶活高 (60 .0 8U/m L)是出发株的 2倍 ,而且其对天然粗纤维分解能力强 ,10 d分解率达 3 5 %。

Separated three strains of cellulose from composting,ordure of horse, soil of gardern and sewage sludge, together with Trichoderma koningii, White rot, Trametes versicolor as original strains. The strains continually mutagenized by ultraviolet radiation, resistant mutants were selected on the producing enzyme medium plate in the presence of glucose. These mutants can shape bigger transparent ring. C - {16 } mutagenized from White-rot mutant can increase 2 times(60.08 U/mL), and the rate of hydrolyzed natural cellulose is about 35% for 10 days.