混合重金属离子相互作用诱导的LPO与CuPb在鲫鱼组织中积累

LPO Accumulation of Copper and Lead and Inducement of Lipid Superoxide by Interaction of Mixture of Metals

采用室内暴露实验方法,研究了混合重金属离子相互作用对Cu、Pb在鱼组织中吸收、积累以及其诱导的脂质过氧化作用。结果表明,混合重金属离子在鱼体内吸收、积累与组织类型有关,Cu、Pb在鱼鳃中受表面位点竞争理论主导,使其积累降低;而在肝脏中受MT诱导作用的影响,积累增高。混合重金属离子诱导的脂质过氧化作用强弱与重金属离子的暴露浓度相关,LPO的产物MDA的含量与混合重金属离子的暴露浓度(0.00—0.54mg·L-1)存在指数关系。用鱼鳃组织的MDA变化作为生物指示物指示环境中存在混合重金属离子的污染敏感于肝脏中的MDA。

The present paper has studied accumulation of copper and lead in fish tissues and lipid superoxide by interaction of the mixture-metals. The results demonstrated that accumulation of copper and lead was related to fish tissues. The accumulation of copper and lead reduced in gill affected mainly by the surface competition theory, and increased in liver by the MT induction theory. The LPO induced by the metal mixture was concerned with its exposure concentration, where the exponential relation was found between MDA (LPO product) content and exposure concentration when the exposure concentration was from 0.00 to 0.54mg/L. The MDA content (LPO product) in the gill that was more sensitive than that in the liver, which may be used as biomark to indicate the pollution of mixture-metals