河南汉族群体D18S51基因座的遗传多态性研究

Genetic polymorphisms of human short tandem repeat D18S51.

目的 研究人类短串联重复序列D18S5 1在河南汉族人群中的遗传多态性 ,并探讨该基因座在法医学和基因诊断中的应用可能性。方法 采集河南无血缘关系汉族个体血样 ,应用Chelex法提取DNA ,聚合酶链式反应扩增 ,非变性聚丙烯酰胺凝胶电泳分型 ,统计学计算。结果 得到D18S5 1在河南汉族群体中的基因频率 ,有 11个等位基因 ,2 9个基因型 ,杂合度为 0 84,个体识别率为 0 98,非父排除率为 0 5 8。结论 在河南汉族人群中D18S5 1基因座多态性较好 ,分布符合Hardy -Weinberg平衡 。

Objective: We researched the genetic polymorphisms of D18S51 in Henan Han population and its using in forensic science.Methods:Collecting not-relative persons in Henan Han population and getting the DNA with Chelex.Using polymerase chain reaction and silver staining to type the persons. Result: A total of 6 alleles and 12 genotypes was found in Henan Han population,its heterozygosity is high and the locus can be used in forensic genetics.Conclusion: We get the frequency of the locus D18S51 in Henan Han population.The results of amniotic fluid,villus,blood stain mean D18S51 is a good locus for forensic.