舌癌脊髓转移细胞系Ts的建立及相关生物学特性

Establishment and characterization of a metastatic cell line from spinal cord metastasis induced by injection of tongue cancer Tb cells in nude mouse

目的 建立舌癌远隔脏器转移的细胞系 ,研究其相关生物学特性。方法 采用瘤细胞裸鼠尾静脉注射法 ,细胞培养法获取舌癌远隔脏器转移细胞系 ,用相差显微镜、HE染色、电镜观察其形态及细胞内部结构 ,染色法显示染色体形态 ,裸鼠背部皮下注射瘤细胞法检验致瘤性并证明其性质。用 2 4孔培养板行生长曲线测定 ,并计算其细胞群体倍增时间 ,用流式细胞仪测定细胞周期来检测其细胞增殖速度。结果 从 3 5只受试裸鼠中获得一只截瘫的裸鼠 ,取其肺、肝、脑和脊髓组织分别行原代培养 ,仅脊髓组织培养获得了稳定传代的细胞系 ,传代 5 0次以上 ,生长稳定 ,Ts倍增时间为 2 6.83h ,S期细胞占 2 5 .5 % ,染色体众数为 60 ;其母细胞Tb倍增时间为 2 8.81h ,S期细胞占 2 1.2 % ,染色体众数为 5 0 ,保持人类染色体形态 ,细胞形态与其母细胞相似 ,裸鼠皮下所致肿瘤组织切片显示为典型的鳞状上皮细胞癌 ,将该细胞命名为Ts。结论 Ts细胞是Tb细胞在裸鼠体内的脊髓转移细胞系 。

Objective To establish a metastatic cell line from distant organ metastasis using tongue cancer Tb cells in nude mice. Methods Tail vein injection of cancer cells and cell culture technic were employed to induce metastasis in distant organ and to obtain cell line from the metastasis cell. counting and flow cytometry were used to study the cell growth, karyotype analysis and histo-pathological observation were used to study the morphology and tumorigenicity.Results Paralized nude mice was observed in 1/35experimental nude mice, the cells derived from the spinal cord were cultured by primary culture and transferred for more than 50 passages, the cells were proved to be of squamouse cell carcinoma from human being by the histopathological features of the tumor induced by the cell injection morphology and karyotype of the cells,the population doubling time and S-phase cell of the cells were 26.83 h and 25.5 % respectively, those of Tb were 28.81 h and 21.2 % respectively, the cell line was named Ts.Conclusion Ts is a metastatic cell line from spinal cord metastasis induced by injection of human tongue cancer Tb cells.