大鼠“内关”穴注射硝酸甘油缓解心肌缺血作用的研究

Study on Injection of Nitroglycerin into Point "Neiguan" for Alleviation of Acute Myocardial Ischemia in Rats

目的 :了解“内关”穴注射硝酸甘油 (NTG)防治急性心肌缺血 (MI)的作用及其机制。方法 :静脉注入垂体后叶素 (PPT)造成MI模型 ,以“内关”注入NTG缓解MI的心电图变化为指标 ,观察保留与切断G6 ～T2 脊神经前后根 (VDS)、以及毁损脊神经节 (DRG)的影响 ,并与舌下运用NTG进行比较。结果 :切断VDS对“内关”注入NTG缓解MI影响不大 ,毁损DRG后MI加重并恢复延迟 ,“内关”比舌下运用NTG防治MI的持续时间更长。结论 :“内关”注射NTG可作为防治冠心病的有效方式之一 ,“内关” DRG 心脏间存在不依赖于中枢的神经短反射通路。

Purpose To investigate the action of injection of nitroglycerin (NTG) into 'Neiguan' (PC 6) on acute myocardial ischemia (MI) and the mechanism. Methods Experimental MI model was produced by intravenous injection of pituitrin. When the ventral and dorsal spinal nerve roots (VDS) at the levels of C 6 T 2 were excised or dorsal root spinal ganglia (DRG) was destroyed at the same levels, the effects of injection of nitroglycerin into 'Neiguan' (PC 6) on changes of ECG were investigated and compared with that of sublingual administration of nitroglycerin. Results There were no significant differences in ischemia feature and recovery of ECG after injection of NTG into 'Neiguan' before and after excision of VDS. However MI was deteriorated and recovery of ECG was delayed after DRG was destroyed. The duration of alleviation of MI caused by administration NTG into 'Neiguan' was longer than that of sublingual administration of NTG. Conclusion Injection of NTG into 'Neiguan' is one of the effective methods for alleviation of MI, and there is a reflex pathway of 'Neiguan' DRG heart which is independent of the central nerve system.