穴位免疫疗法的实验研究——不同注射方法对环磷酰胺造模大鼠免疫功能的影响

Experimental Study on Acupoint Immunotherapy--Effects of Different Injection Methods on Immunologic Functions in Cyclophosphamide Model Rats

目的 :通过观察不同途径注射基因重组白细胞介素 2 (rIL 2 )对环磷酰胺造模大鼠免疫功能的影响 ,为临床应用穴位免疫疗法提供依据。方法 :采用环磷酰胺造模大鼠 ,以大鼠胸腺、巨噬细胞吞噬百分率和吞噬指数、血清IL 2含量为指标 ,通过穴位注射、肌肉注射rIL 2及穴位注射生理盐水进行疗效比较。结果 :3种治疗方法均能显著提高环磷酰胺造模大鼠降低的巨噬细胞吞噬百分率和吞噬指数、血清IL 2含量 (P <0 0 5 ,P <0 0 0 1) ,以穴位注射rIL 2组指标改善意义最显著 (P<0 0 0 1) ;但 3种方法对造模大鼠降低的胸腺指数的提高无统计学意义。结论 :穴位注射rIL 2疗效优于肌肉注射rIL 2。

Purpose To observe effects of different injection methods of gene recombined interleukin 2(rIL 2) on immunologic functions in the cyclophosphamide model rats so as to provide a basis for clinical application of acupoint immunotherapy. Methods In the cyclophosphamide model rats, the thymus, macrophage phagocytic percent and phagocytic index, serum IL 2 content were used as indexes to compare the therapeutic effects of acupoint injection of rIL 2, intramuscular injection of rIL 2 and acupoint injection of saline. Results The 3 injection methods all could significantly increase the decreased macrophage phagocytic percent, phagocytic index and serum IL 2 content in the model rats ( P <0 05 and P <0 001 respectively), and more markedly in the rIL 2 acupoint injection group ( P<0 001 ); and no significant improvement of the thymus index was found for the three injection methods. Conclusion The therapeutic effect of acupoint injection of rIL 2 is better than that of intramuscular injection of rIL 2.