甲氧沙林对人毛囊黑素细胞增殖、活化和黑素合成作用的研究

Effects of 8-methoxypsoralen on Proliferation, Activation and Melanin Synthes is of Human Hair Follicle Melanocytes

目的研究甲氧沙林对人毛囊黑素细胞活化、增殖和黑素合成的影响。方法体外培养正常人毛囊黑素细胞,观察不同浓度甲氧沙林(10～500μmol/L)对毛囊黑素细胞形态、增殖、酪氨酸酶活性和黑素合成的影响。结果50μmol/L甲氧沙林处理细胞7d后,毛囊黑素细胞树突明显增多延长,胞浆内出现较多棕褐色颗粒,酪氨酸酶活性和黑素含量增加显著高于对照组(P<0.01)。结论甲氧沙林能诱导毛囊黑素细胞树突增多延长,酪氨酸酶活性和黑素含量增加。

Objective To study the effects of 8-methoxypsoralen (8-MOP) on a ctivation, proliferation and melanin synthesis of hair-follicle melanocytes. Me thods The cultured human hair follicle melanocytes were treated with various con centrations of 8-MOP(10～500 ìmol/L) in vitro. The effects of 8-MOP were obse rved on cellular morphologic changes, proliferation, tyrosinase activity and mel anin contents of cultured melanocytes. Results Seven days after treatment with 8-MOP(50 ìmol/L), striking changes were found in cellular morphology with many elongated dendrites, and deposition of brown granules in cytoplasm. Tyrosinase a ctivity and melanin contents also remarkably increased in treated cells compared to those in untreated cells(P< 0.01). Conclusion 8-MOP can induce proliferatio n, tyrosinase activity and melanin synthesis of human hair follicle melanocytes.