摘要
day-old AA broilers were conducted with violent Marek′s disease virus (vMDV). The activity of SOD, Se-GSHPx, content of LPO in central, peripheral immune organs and main visceral organs of chicks were detected at 7, 14, 28, 42, and 56 days after infection with vMDV, respectively, and chemilunescence (CL) assay was adopted to detect the ability of active oxygen radical (AOR) generation of lymphocytes from thymus, spleen and bursa of Fabricious. The results showed: (1) the activity of total SOD (T-SOD) and Mn-SOD of vMDV-infected chicks decreased significantly (P<0.05, P<0.01) compared to control group, whereas that of Cu, Zn-SOD increased slightly (P>0.05); (2) the activity of Se-GSHPx decreased significantly (P<0.05, P<0.01); (3) the content of LPO increased significantly (P<0.05, P<0.01); (4) the value of CL of lymphocytes increased significantly (P<0.05, P<0.01. These showed that vMDV infection resulted in increasing of generation of AOR and decreasing of activity of AOR scavenging enzyme system, leading to deposition of AOR, intensive lipid peroxidation and conversion of cells in chicks. Which gave a further proof that dynamic changes of detected items were consistent with action of vMDV. These proved that AOR resulting from vMDV infection mediated Marek′s disease (MD) and MD oncoma.
day-old AA broilers were conducted with violent Marek′s disease virus (vMDV). The activity of SOD, Se-GSHPx, content of LPO in central, peripheral immune organs and main visceral organs of chicks were detected at 7, 14, 28, 42, and 56 days after infection with vMDV, respectively, and chemilunescence (CL) assay was adopted to detect the ability of active oxygen radical (AOR) generation of lymphocytes from thymus, spleen and bursa of Fabricious. The results showed: (1) the activity of total SOD (T-SOD) and Mn-SOD of vMDV-infected chicks decreased significantly (P<0.05, P<0.01) compared to control group, whereas that of Cu, Zn-SOD increased slightly (P>0.05); (2) the activity of Se-GSHPx decreased significantly (P<0.05, P<0.01); (3) the content of LPO increased significantly (P<0.05, P<0.01); (4) the value of CL of lymphocytes increased significantly (P<0.05, P<0.01. These showed that vMDV infection resulted in increasing of generation of AOR and decreasing of activity of AOR scavenging enzyme system, leading to deposition of AOR, intensive lipid peroxidation and conversion of cells in chicks. Which gave a further proof that dynamic changes of detected items were consistent with action of vMDV. These proved that AOR resulting from vMDV infection mediated Marek′s disease (MD) and MD oncoma.
作者
Li Qingzhang, Hao Yanhong, Chi Yujie, Zhong Pi, Gao Xuejun, Wei Ping, Yu Hongxiang, Xu Baorong, Ge Ming, Qu Qihuan (Department of Animal Physiology and Biochemistry, Northeast Agricultural University, Harbin 150030, PRC)
