欧洲甜樱桃砧木‘SL64’离体叶片再生体系研究

Studies on efficient plant regeneration system from in vitro leaves of sweet cherry rootstock 'SL64'

【目的】探明欧洲甜樱桃砧木‘SL64’离体叶片再生技术体系。【方法】以‘SL64’的组培苗叶片为试材,从基本培养基、激素配比、暗培养和叶片生理状态等方面对影响离体叶片再生的关键因素进行了研究,建立了SL64砧木离体叶片的高效再生体系。【结果】‘SL64’采用改良DKW培养基为基本培养基再生效果最好,显著优于MS、WPM和QL培养基;最佳激素组合为6-BA 2.5 mg·L-1+IBA 0.05 mg·L-1;接种后5℃下暗培养5 d、然后23℃下暗培养10 d可明显提高愈伤组织发生率和不定芽再生率。【结论】通过以上条件的优化,建立了‘SL64’的高效离体再生技术体系,离体叶片不定芽再生率为90%左右,平均每叶再生不定芽数达5~6芽。

【Objective】 The objective of the study was to establish the efficient plant regeneration system from in vitro leaves of sweet cherry rootstock ‘SL64'. 【Method】 In vitro young leaves of sweet cherry rootstock ‘SL64' were used as explants to study the effect of different basic media, hormone combinations,dark cultivation and leaf physiological status on the adventitious shoot regeneration. 【Result】 The results showed that the modified DKW medium was the optimal basic culture medium for the adventitious shoot regeneration and subculture. Modified DKW was superior to MS, WPM and QL medium. The best hormone combination was 6-BA 2.5 mg·L-1and IBA 0.05 mg·L-1. The callus formation and adventitious shoot regeneration were improved when the explants were cultured for 5 days at 5 ℃ and then for 10 days under the dark condition after the explants were transferred to the induction medium. 【Conclusion】 Under the above condition,the adventitious shoot regeneration rate from in vitro leaves was 90% and the adventitious shoot per explants were up to 5 to 6.