80个梨品种SSR特征指纹数据表的构建

Construction of SSR characteristic fingerprinting data table for 80 pear cultivars

【目的】通过鉴定80份梨种质资源SSR指纹数据,构建梨品种指纹图谱数库表,为实现梨种质资源鉴定及新品种DUS测试的可视化和简便化奠定数据基础。【方法】利用荧光标记技术检测SSR位点,并对SSR位点基因数目进行统计,SSR位点排序,并对供试品种进行排序区分和数据标示,构建了80个梨品种的特征指纹数据表。【结果】12对引物中,可利用NH039A、NH007b、NAUpy97a、NB105a、NB141b、NAUpy82r 6对引物区分所有供试品种,可作为区分这80个梨品种的核心引物。【结论】筛选出了区分供试80个梨品种的核心引物,为梨指纹图谱数据库的共建和共享提供了数据基础。

【Objective】The SSR fingerprinting data of 80 pear cultivars were collected and the characteristic fingerprint data table was constructed.The data foundation was established for achieving visualization and convenience of fruit germplasm identification and new cultivars DUS testing.【Methods】The fluorescent labeling of simple sequence repeat(SSR)detection was used for SSR loci detection,and the SSR genotype number of every locus was calculated.Then the order of SSR loci was adjusted and the cultivars were sorted and distinguished.Finally,the cultivars characteristic fingerprint data were colored,a new SSR characteristic fingerprint data table of 80 cultivars was established.【Results】The results showed that six primer pairs(NH039A,NH007 b,NAUpy97 a,NB105 a,NB141 b and NAUpy82r)among 12 SSR primer pairs used in this study could entirely distinguish all 80 pear cultivars.These six primer pairs can be used as a set of core primer pairs to identify all 80 pear cultivars.【Conclusion】The core SSR primers for80 pear cultivars identification were selected,and it provide data for sharing the fingerprinting data and co-constructing the fingerprint database.