山东地区梨腐烂病菌的分离鉴定

The identification and the Pathogenicity of analysis for the pathogen of pear Valsa canker

【目的】获得梨腐烂病的致病菌株用于后期筛选抗病梨种质资源。【方法】采集具有典型腐烂病症状的‘黄金梨’枝干样品进行组织分离、培养,获得梨腐烂病菌株,采用形态学、分子生物学以及病原菌致病性相结合的方法对其进行鉴定。【结果】根据分离菌株的菌落、分生孢子形态特征、rDNA-ITS序列分析将分离到Va1菌株鉴定为腐烂菌(Valsa mali),并选取8个山东地区的梨种质资源进行致病性验证。【结论】分离得到的菌株对8个品种都具有致病性,证实为梨腐烂病的病原。

【Objective】In order to detect the resistance of pear germplasms to canker,we obtained fungal pathogens from pear trees.【Methods】Isolates from disease-affected branches of pear were purified culture and obtaining pear rot disease strain named(No.Va1).Identification of the pathogenic fungus was carried out according to the characteristics of fungal morphology,pathogenicity and by rDNA-ITS sequence analysis.【Results】The strain was identified as Valsa mali based on the colony and conidiophores morphological observation and the rDNA-ITS sequence homology.Eight pear germplasm resources were selected for pathogenicity detection.【Conclusion】The results showed that the isolate was the pathogen of pear rot.It has certain pathogenicity of the Va1 isolate on eight varieties of pear.