泰山磐石散加味方对先兆流产模型大鼠血清孕酮、β-人绒毛膜促性腺激素水平的影响

Influence of modified Taishanpanshi San on serum progesterone andβ-human chorionic gonadotrophin in model rats with threatened abortion

目的研究泰山磐石散加味方对先兆流产模型大鼠血清孕酮(P)、β-人绒毛膜促性腺激素(β-HCG)含量的影响,从而探讨其在先兆流产治疗中的作用机制。方法将32只已孕Wistar大鼠按随机数字表法分为4组,分别为空白对照组、模型组、泰山磐石散加味方低剂量组(简称低剂量组)、泰山磐石散加味方中剂量组(简称中剂量组),分别灌胃蒸馏水及泰山磐石散加味方煎液。以米非司酮复制先兆流产大鼠模型。于大鼠妊娠第16天股动脉采血,ELISA法测定血清P和β-HCG含量,观察胚胎丢失情况并计算流产率。结果与空白对照组比较,模型组血清P和β-HCG含量均显著降低,流产率显著升高,2组比较差异有统计意义(P<0.05或P<0.01),提示造模成功。与模型组比较,低剂量组、中剂量组血清P和β-HCG含量均显著升高,流产率均显著降低,差异有统计意义(P<0.05或P<0.01),且中剂量组的作用优于低剂量组(P<0.05)。结论泰山磐石散加味方能有效升高血清P和β-HCG含量,从而达到维持妊娠、促进宫腔内胚胎生长发育、避免流产的目的。

Objective To study the influence of modified Taishanpanshi San on the serum progesterone( P)and β-human chorionic gonadotrophin( β-HCG) in model rats with threatened abortion and explore the mechanism in the therapeutic process. Methods 32 Wistar rats were randomly divided into control group,model group,lowdose treatment group and moderate-dose treatment group. They all took distilled water and the decoction intragastrically. Model rats were reproduced by mifepristone. Blood was drawn via femoral artery on the 16 thday of pregnancy.The contents of P and β-HCG were determined by ELISA. Lost embryos were observed and abortion ratio was calculated. Results Compared with control group,the contents of P and β-HCG in model group decreased ob- v iously,abortion ratio increased highly. There was a significant difference( P < 0. 05 or P < 0. 01). Compared with model group,the contents in two treatment groups increased,while the abortion ratio was decreased,there was a significant difference( P<0.05 or P<0.01). The effect of moderate-dose group was better than the low-dose group. Conclusion Modified Taishanpanshi San can subsist pregnancy,stimulate growth of embryo and avoid abortion by raising the contents of P and β-HCG.