痰瘀同治方对阿尔茨海默病小鼠脑组织氧化应激与神经炎性反应的影响

Influence on oxidative stressin brain and neural inflammatory reaction of mouse with Alzheimer's disease by using Tanyu Tongzhi Fang

目的观察痰瘀同治方对阿尔茨海默病(AD)小鼠学习认知能力、脑组织氧化应激和神经炎性反应的影响。方法将100只雄性小鼠随机分为5组:对照组,模型组,痰瘀同治方高、中、低剂量组,每组20只。除对照组外,在其他各组小鼠侧脑室注射β淀粉样肽(Aβ)1-40,建立AD小鼠模型,连续14周给予相应药物灌胃。采用Morris水迷宫试验观察小鼠行为学的改变。麻醉后处死小鼠,采用比色法测定小鼠脑组织内丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)的含量及活性,免疫组织化学法测定脑内神经胶质元纤维酸性蛋白(GFAP)阳性细胞(星形胶质细胞)的表达。结果与模型组相比,痰瘀同治方可以明显改善小鼠学习认知能力(P<0.01),降低脑组织内MDA水平(P<0.05),增加SOD,GSH-PX和GSH含量及活性(P<0.05),降低星形胶质细胞表达数量(P<0.05)。结论痰瘀同治方通过抑制AD小鼠脑组织内氧化应激与神经炎性反应,明显改善AD小鼠的学习认知能力。

Objective To observe the influence on the learning cognitive ability,oxidative stress in brain and neural inflammatory reaction of mouse with Alzheimer's disease( AD) by using Tanyu Tongzhi Fang. Methods100 male mice were randomly divided into control group,model group,high-dosage,medium-dosage and low-dosage treatment groups,20 cases each. Except control group,mice in other groups were injected with β-AP on lateral ventricle to make model group,intervened by intragastric administration accordingly for 14 weeks. Ethological changes in mice were observed through Morris water maze test. After execution by anesthesia,the content and activity of MDA,SOD,GSH,GSH-PX in mice's brain were determined by colorimetry. GFAP positive cellular expression( astrocyte) in brain was determined by immunohistochemical method. Results Compared with m odel group,Tanyu Tongzhi Fang greatly improved the mice's learning cognitive ability( P<0.01),decreased the level of MDA in brain( P<0.05),raised the content and activity of SOD,GSH-PX,GSH( P<0.05),decreased the expression amount of astrocyte( P<0.05). Conclusion Tanyu Tongzhi Fang can r emarkably improve the learning cognitive ability of AD mice by inhibiting the oxidative stress in brain and neural inflammatory reaction.