石榴健胃片对脾肾阳虚型溃疡性结肠炎模型大鼠结肠炎症因子的影响

Effects of Shiliu Jianwei Tablet on inflammatory factors in the model rats with ulcerative colitis due to yang-deficiency of spleen and kidney

目的研究石榴健胃片对脾肾阳虚型溃疡性结肠炎(SKD-UC)模型大鼠结肠肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-6和IL-10的影响。方法采用15%大黄混悬液灌胃联合2,4,6-三硝基苯磺酸-乙醇溶液灌肠的病证结合法建立SKD-UC大鼠模型,将模型大鼠随机分为阳性组,模型组,石榴健胃片高、中、低剂量组,每组20只,另取20只作为空白组。造模成功后第2天开始进行药物干预,阳性组大鼠灌胃柳氮磺胺吡啶(SASP,0.36g/kg),石榴健胃片高、中、低剂量组分别灌胃石榴健胃片混悬液2.4,1.2,0.6g/kg,模型组和空白组灌胃等体积生理盐水,每天1次,连续21d。末次给药后24h处死大鼠,取结肠组织,采用逆转录-聚合酶链反应(RT-PCR)法检测结肠组织中IL-1β,IL-6,IL-10,TNF-α的基因表达。结果与空白组比较,模型组大鼠结肠IL-1β,IL-6,TNF-α的mRNA相对表达量明显增高,IL-10的mRNA相对表达量明显降低,组间比较差异均有高度统计意义(P<0.01);与模型组比较,石榴健胃片高、中、低剂量组大鼠结肠IL-1βmRNA和TNF-αmRNA相对表达量明显降低(P<0.05或P<0.01),高、中剂量组IL-6mRNA相对表达量明显降低(P<0.01),而高、中、低剂量组IL-10mRNA相对表达量明显升高(P<0.05或P<0.01);与阳性组比较,石榴健胃片高剂量组大鼠结肠IL-1β,TNF-α和IL-6的mRNA相对表达量及中剂量组IL-1βmRNA相对表达量明显降低(P<0.05),高、中剂量组IL-10mRNA相对表达量明显升高(P<0.05);各剂量组之间IL-1β,IL-6,TNF-α和IL-10的mRNA相对表达量比较差异均无统计意义(P>0.05)。结论石榴健胃片对SKD-UC模型大鼠的结肠TNF-α,IL-1β,IL-6及IL-10的基因表达具有调节作用。

Objective To study the effects of Shiliu Jianwei Tablet on TNF-α,IL-1β,IL-6and IL-10 in the model rats with ulcerative colitis(UC)due to yang-deficiency of spleen and kidney.Methods UC rat models were created by intragastric administration of rhubarb powder suspension(15%)combined with enema of 2,4,6-trinitro-benzene-sulfonic acid(TNBS)/ethanol.Wistar rats were randomly divided into blank group(normal saline),positive control group(SASP,0.36g/kg),model group(normal saline),high-(2.4g/kg),middle-(1.2g/kg)and low-dose(0.6g/kg)groups interfered with Shiliu Jianwei Tablet,20 in each group.The treatment was carried out once a day and lasted for 21 days in succession.Then the rats were put to death and colon tissues were extracted.The gene expressions of TNF-α,IL-1β,IL-6and IL-10 were detected by RT-PCR.Results Compared with blank group,the mRNA of IL-1β,IL-6,TNF-αin model group increased obviously while IL-10decreased(P <0.01).Compared with model group,the mRNA of IL-1β,TNF-αin the 3dose-groups decreased(P<0.05 or P<0.01);the mRNA of IL-6in high-and middle-dose groups decreased obviously(P<0.01),while the mRNA of IL-10 in the 3dose-groups increased(P<0.05 or P<0.01).Compared with positive control group,the mRNA of IL-1β,TNF-αand IL-6in high-dose group as well as the mRNA of IL-1βin middle-dose group decreased(P<0.05),the mRNA of IL-10 in high-and middle-dose groups increased(P<0.05).There was no any statistic difference among dose-groups(P>0.05).Conclusion Shiliu Jianwei Tablet can play a regulation role on the gene expression of TNF-α,IL-1β,IL-6and IL-10 in UC rats due to yang-deficiency of spleen and kidney.