气候因素对Ⅱ型胶原蛋白所致类风湿关节炎模型大鼠关节炎症、免疫脏器指数及组织形态的影响

Influence of climatic factor on the joint inflammation,immune viscera index and morphology of model rats with rheumatoid arthritis induced by Ⅱ-collagen

目的观察气候因素对Ⅱ型胶原蛋白所致类风湿关节炎(RA)模型大鼠关节炎症、免疫脏器指数及组织形态的影响。方法将40只SD大鼠随机分为空白组、Ⅱ型胶原组、Ⅱ型胶原+人工风寒湿组(简称风寒湿组)、Ⅱ型胶原+人工风湿热组(简称风湿热组)4组,每组10只,雌雄各半。空白组给予等量生理盐水,Ⅱ型胶原组皮下注射Ⅱ型胶原佐剂混合液,其余2组采用Ⅱ型胶原佐剂混合液诱导,外加人工气候因素制备RA大鼠模型。造模28 d后,比较各组大鼠的关节炎评分、胸腺指数、脾脏指数,并观察各组大鼠左后肢踝关节的组织形态改变。结果与空白组比较,各模型组大鼠均出现了不同程度的足趾关节肿胀,关节炎评分均显著升高,差异有高度统计意义(P<0.01);各模型组脾脏指数均有所升高,其中风寒湿组与空白组比较差异有统计意义(P<0.05);组织形态观察,造模后,Ⅱ型胶原组、风寒湿组和风湿热组大鼠踝关节出现不同程度的滑膜增生、淋巴细胞浸润。结论气候因素对Ⅱ型胶原蛋白所致RA模型大鼠关节炎症、组织形态具有一定的影响,能加重RA大鼠关节的病理损伤。

Objective To observe the influence of climatic factor on the plantar form and immune viscera in-dex of model rats with rheumatoid arthritis(RA)induced by Ⅱ-collagen.Methods 40 SD rats(half male andhalf female)were randomly divided into four groups:normal control group,Ⅱ-collagen group,Ⅱ-collagen+simula-ted factor of wind-cold-dampness group(shortened as WCD group)and Ⅱ-collagen+simulated factor of rheumaticfever group(shortened as rheumatic fever group),ten in each group. The normal control group was given normal sa-line at same dose. Ⅱ-collagen group was intervened with the adjuvant mixture of Ⅱ-collagen. The other two groupswere induced by adjuvant mixture of Ⅱ-collagen plus simulated climatic factors to get RA rat model. On 28 thdayafter model making,arthritis score,thymus index and spleen index were compared,the histopathological changes ofthe left-behind foot plantar in all groups were observed.Results Compared with the normal control group,theother 3 model groups had plantar joint swelling in various degree,arthritis score increased significantly and there wasa statistically significant difference( P< 0. 01). The spleen indexes of model groups increased,meanwhile thespleen index of WCD group was significantly greater than that of normal control group,there was a obvious differ-ence(P<0. 05). Ankle synovial hyperplasia and lymphocytic infiltration were found in three model groups.Conclusion Climatic factors have certain influence on the joint inflammation and morphology of RA rats induced byⅡ-collagen,also can aggravate the joint pathological lesion.