不同产地黄芩对发热模型大鼠体温及血清中IL-1β、PGE_2的影响

Effects of HuangQin from Different Producing Areas on Body Temperature,the Levels of IL-1β and PGE_2 of Rats with Fever

目的:研究不同产地黄芩水煎液对脂多糖(LPS)诱导的发热模型大鼠的解热作用及机制。方法:将筛选合格的80只大鼠随机分为8组,即生理盐水组、模型组及河北承德、陇西、渭源产黄芩的高、低剂量组。黄芩水煎液灌胃给药2天后,LPS腹腔注射诱导大鼠发热模型,再灌胃给药1天。检测各组大鼠在不同时间点的升温值及血清中IL-1β、PGE2的含量。结果:与生理盐水组比较,模型组大鼠的升温值、IL-1β、PGE2含量显著升高(P<0.05);各给药组大鼠在不同时间点的升温值及IL-1β、PGE2含量显著低于模型组(P<0.05);同一产地黄芩高剂量组大鼠在2、3、4小时的升温值及血清中IL-1β、PGE2含量显著低于低剂量组(P<0.05);不同产地高剂量组间比较,在3、4小时时河北承德组大鼠升温值显著低于陇西、渭源组,河北、渭源组IL-1β含量显著低于陇西组,河北、陇西组PGE2含量显著低于渭源组(P<0.05)。结论:不同产地黄芩水煎液均有显著的解热作用,河北承德黄芩水煎液的解热效果优于甘肃陇西、渭源黄芩,高剂量组解热效果优于低剂量组;抑制IL-1β、PGE2的释放可能是黄芩发挥解热作用的机制之一。

Objective: To study antipyretic effects and the mechanism of the decoction of HuangQin(Scutellaria baicalensis Georgi) from different producing areas on lipopolysaccharide(LPS)-induced rat model with fever.Methods: Eighty rats were randomized into eight groups: physiological saline group, the model group, high and low doses groups of HuangQin from Chengde, Longxi and Weiyuan. After intragastric administration of HuangQin decoction for two days, the rat model with fever was induced by peritoneal injection of LPS, and they received intragastric administration for one day. The contents of IL-1β and PGE_2 and the temperature rise at different times of the rats in different groups were detected. Results: Compared with physiological saline group, the contents of IL-1β and PGE_2 and the temperature rise notably raised in the model group(P<0.05); these indexes at different times in the medication groups were lower than these of the model group significantly(P<0.05); the contents of IL-1β and PGE_2 and the temperature rise in two hours, three hours and four hours in the high dose group of HuangQin from the same producing area were lower than these of the low dose group apparently(P<0.05); the comparison among high doses groups of HuangQin from different producing areas showed that the temperature rises at three and four hours in Chengde group were notably lower than these of the Longxi group and Weiyuan group, the contents of IL-1β in Chengde group and Weiyuan group were lower than these of Longxi group notably, the contents of PGE_2 in Chengde group and Longxi group were lower than these of Weiyuan group significantly(P<0.05). Conclusion: The decoction of HuangQin from different producing areas presents notable antipyretic effects, antipyretic effects of HuangQin from Chengde are superior to these of HuangQin from Longxi and Weiyuan, the effects of high dose group are superior to these of low dose groups; inhibiting the release of IL-1β and PGE_2 might be one of the mechanisms of HuangQin bringing antipyretic effects into play.