寄主植物对棉蚜乙酰胆碱酯酶的影响

Influence of host plants on the acetylcholinesterase activity of cotton aphid

通过生物测定和生化分析研究了5种不同寄主植物上的棉蚜种群对灭多威的敏感性、乙酰胆碱酯酶(AChE)活性及灭多威对AChE的抑制作用。结果发现,取食石榴和花椒的棉蚜AChE活性最高,是冬青上棉蚜(最低)的2.00倍和3.33倍,说明寄主植物可以影响棉蚜AChE的活性。灭多威对不同寄主植物上的棉蚜个体的AChE进行抑制后,发现棉花和冬青上的棉蚜种群中AChE对灭多威不敏感的棉蚜个体多于花椒、木槿和石榴上的棉蚜种群,这与棉花及冬青上的棉蚜对灭多威耐药性较其它三种强的趋势近似,表明AChE敏感度的降低是棉蚜对灭多威产生抗药性的原因之一。

By bioassay and biochemical analyses, the susceptibility to insecticide of cotton aphid Aqhis gossypii Glover populations on Chinese prickly ash , hibiscus, pomegranate, Chinese ilex and cotton collected from Gaomi of Shan Dong Province, the activities of their acetylcholinesterase (AChE ) and the inhibition by methomyl on AChE activity were tested. The results showed that the AChE activities of cotton aphid fed on pomegranate and Chinese prickly ash were 2-fold and 3.33-fold compared with cotton aphid on pomegranate, respectively. It was suggested that the difference in the AChE activity of cotton aphid might be involved in different host plants which aphid fed on. The population of aphids fed on cotton and pomegranate had more individuals with unsusceptible AChE to methomyl than those aphids fed on Chinese prickly ash, hibiscus and pomegranate. This above had the similar issue with that the aphid population fed on cotton and Chinese ilex had higher resistance to methomyl than other populations fed on other three host plants. The results showed that the unsusceptible AChE might be an important role in the resistance of cotton aphid against methomyl.