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单纯疱疹病毒糖蛋白单克隆抗体对致死性腹腔感染BALB/c幼鼠的被动保护作用 被引量:2

Protection against Lethal Challenge of BALB/c Mice by Passively Transferred Monoclonal Antibodies to Glycopro-teins of Herpes Simplex Virus
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摘要 用单纯疤疹病毒(HSV)Ⅰ型SM44株和Ⅱ型SaV株分别腹腔感染BALB/c小鼠,于感染前后不同时间经腹腔注射HSV单克隆抗体(McAb)观察6株McAbs对致死性腹腔感染小鼠的被动保护作用。结果4株McAbs(2C5、1A12、Mad-2、1D10)对HSV-Ⅰ感染的小鼠有保护作用,5株McAbs(2C5、1A12、2A8、1D10、CH-A9)对HSV-Ⅱ感染的小鼠有保护作用。体内保护作用与体外的中和活性相关;并分析了McAbs在中和试验中有无补体参与条件下的保护能力。还证实了HSV糖蛋白在急性感染病程中其型特异性和型共同性抗原决定簇在体内的表达。 BALB/c mice were intraperitoneally challenged with the SM44 strain of HSY-1 and the say strain of HSV-2, respectively. At the time of challenge or 24 hou- rs later, 6 monoclonal antibodies directed against glycoproteins of herpes simplex virus were administered intraperitoneally for evaluation of their passive protection of the animals against lethal challenge. It was found that 4 McAbs ( 2C5, 1A12, Mad-2 and 1D10.) might protect against HSV-1 infection while 5 McAbs ( 2C5, 1A12, 2A8, 1D10 and CH-A9 ) might protect against HSV-2 infection. A direct correlation was observed between in vitro neutralization and in vivo protection, protection against HSV infection by the monoclonal antibodies was analyzed by neutralization tests with or without complement participation. The results confirmed that the glycoproteins of herpes simplex virus expressed both type-common and type-specific determinants during the course of acute virus infection in vivo.
出处 《单克隆抗体通讯》 CSCD 1992年第1期14-18,共5页
关键词 单纯疱疹病毒 糖蛋白类 单克隆抗体 herpes virus hominis glycoproteins antibodies, monoclonal passive protection
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参考文献3

  • 1喻启桂,秦克锋,高谦,汪美先,姜绍谆,王海涛.放射免疫沉淀试验鉴定单纯疱疹病毒糖蛋白C[J].中国免疫学杂志,1990,6(6):333-337. 被引量:3
  • 2高谦,姜绍谆,马文煜,钟访英,蔡晓宁,汪美先.抗HSV-1淋巴细胞杂交瘤的建立及应用单克隆抗体对HSV分型[J]病毒学杂志,1986(04).
  • 3高谦,沈茜,汪美先.产生抗单纯疱疹病毒单克隆抗体杂交瘤细胞系的建立[J]解放军医学杂志,1985(02).

二级参考文献1

  • 1王海涛,徐志凯,汪美先.酶标记抗人IgM单克隆抗体的制备及其初步鉴定与应用[J]第四军医大学学报,1987(06).

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