采用Ⅳ型胶原酶构建大鼠脑出血模型

Establishment of rat cerebral hemorrhage model by type Ⅳcollagenase injection

目的 利用Ⅳ型胶原酶构建大鼠脑出血模型 ,并研究血肿形成速度和血肿形成大小的影响因素。方法 使用健康雄性 2 0 0～ 2 5 0gWistar大鼠 ,随机分组 ,分别脑内尾状核注射不同容积Ⅳ型胶原酶 肝素生理盐水溶液、单纯Ⅳ型胶原酶生理盐水溶液、单纯生理盐水 ,于术后 6h、12h、2 4h、3d、7d分别观察冰冻切片连续切片血肿大小。结果 单纯生理盐水注射仅引起 12h内的针道渗血 ,单纯胶原酶生理盐水注射 3d可以形成直径 3mm左右的血肿 ,Ⅳ型胶原酶 肝素生理盐水注射 2 4h可形成直径 3mm左右的血肿。结论 Ⅳ型胶原酶 肝素生理盐水注射可以建立理想稳定的脑出血动物模型 。

Objective To establish a cerebral hemorrhage model in rats by local injection of type Ⅳcollagenase, and explore the factors affecting the speed and volume of hematoma formation Methods A total of 150 healthy male Wistar rats weighing from 200 to 250 g were randomly divided into 3 groups, control, collagenase, and collagenase/heparin groups Animals in those groups received injection of saline, collagenase (0 2 U/μl), and collagenase (same dose) and heparin (2 U/μl) with different volume respectively at the caudate nucleus The volume of the hematoma in the rats ( n =6 at each time point) was observed 6, 12, and 24 h, and 3, 7d after the injection Results Permeation of blood was found in 12 h after injection in control group Hematoma about 3 mm in diameter was found in 3 d after injection in collagenase group, while in collagenase/heparin group, it was found in 24 h Conclusion Cerebral hemorrhage model established by local injection of collagenase/heparin in saline solution is ideal and reliable, and the size of hematoma is in correlation with the volume of solutions injected into the brain