大鼠不稳定膀胱与MR亚型密度改变关系的实验研究

Relationship between muscarinic receptor subtype density change and unstable bladder in rats

目的 探讨毒蕈碱受体 (MR)亚型密度变化与不稳定膀胱发生之间的关系。方法 建立不稳定膀胱大鼠模型 ,采用RT PCR方法测定不稳定膀胱及对照组逼尿肌毒蕈碱受体 (MR)亚型密度。结果 对照组和不稳定膀胱组逼尿肌组织均含有m2 RNA和m3RNA ,没有检测到m1 、m2 和m3亚型的mRNA。正常对照组膀胱逼尿肌m2 RNA(1 67± 0 42 )含量明显多于m3RNA(0 64± 0 19) ,两者之比约 2 5 9∶1;不稳定膀胱逼尿肌中m2 和m3密度均增加 ,分别为 (2 0 3± 0 65 )和 (1 5 3± 0 46) ,m3增加更明显 (P <0 0 1) ,两者比值显著缩小 ,约 1 4∶1。结论 不稳定膀胱产生与MR亚型密度上调及M2 R、M3R亚型之间的比例失调有关。

Objective To study the relationship between muscarinic receptor(MR) subtype density changes and the producing of unstable bladder Methods The rat model of unstable bladder was established and the MR subtype density of control and unstable bladders were defermined by RT-PCR Results There were only m 2RNA and m 3RNA in the detrusor of control and unstable bladders, but no m 1, m 2 and m 3 mRNA, the amount of m 2RNA(1 67±0 42)was larger obviously than m 3RNA(0 64±0 19),the ratio was 2 59∶1 in control group, and the density of both m 2 and m 3 in unstable bladder increased to (2 03±0 65) and(1 53±0 46)respectively, and the increase of m 3 was more significant ( P <0 01) with the ratio of 1 4∶1 Conclusions MR subtype up regulation and the ratio unbalance of MR subtype are closely related to the producing of unstable bladder