基于葡萄糖氧化酶-辣根过氧化物酶催化体系的葡萄糖SERS定量分析研究

SERS of Perylenedimide Derivative based on Ag@TiO_2 Substrates

葡萄糖氧化酶法(glucose oxidase,GOx)是生物体系以及葡萄糖检测中常用的方法,其中葡萄糖氧化酶可以专一地催化葡萄糖生成葡萄糖酸以及过氧化氢(H2O2)。葡萄糖氧化酶内部的黄素腺嘌呤二核苷酸(FAD)则是其主要的活性成分,在葡萄糖的催化氧化过程中起着电子传递的作用。传统的葡萄糖氧化酶法主要是通过借助辣根过氧化物酶(horse radish peroxidase,HRP)/TMB显色体系完成对葡萄糖的定量分析,即葡萄糖氧化酶可以特异地催化氧化葡萄糖,产生H2O2。在H2O2存在的体系中,辣根过氧化物酶可以将底物分子四甲基联苯胺(TMB)催化氧化。表面增强拉曼光谱(surface-enhanced Raman scattering,SERS)是一种超灵敏检测手段并且被广泛应用于生物检测中[1]。经研究发现,TMB分子具有很高的SERS活性,并且TMB分子经过催化反应之后,其SERS光谱发生明显的增强,我们以此实现葡萄糖的定量分析研究。

Glucose oxidase method is a kind of very useful and important enzyme-catalyzed reaction for the quantitative analysis of glucose.The main procedure was as follows:the GOxcan catalytical oxidase glucose with the produce of H2O2.Horseradish Peroxidase(HRP)can catalytic oxidize TMB to its oxidation state,and the SERS signal of TMB and its oxidation product shows difference.The quantitative analysis of glucose can be thus proposed by monitoring the SERS intensity change of TMB molecule.