人体胆汁及胆囊癌组织的同步辐射显微红外光谱研究

Synchrotron FTIR Microspectroscopy Investigation of Human Bile and Gallbladder Carcinoma Tissue

同步辐射红外光源的亮度是普通光源的100~1 000倍,在测试微小样品时具有更高的空间分辨率和信噪比。本文利用同步辐射显微红外比较了患有良性和恶性肿瘤病人的胆汁样品,在测试过程中将新鲜胆汁滴在基片上,能无损测试样品。通过比较发现患良性肿瘤病人的胆汁样品中具有更高含量的脂类,而患恶性肿瘤病人的胆汁样品则含有较高含量的磷酸脂,且两者含有的胆红素和蛋白质在组成和结构上有较大的差异。同时,利用同步辐射显微红外对患胆囊癌病人的癌症组织和癌旁组织进行微区成像,并且利用层次聚类分析可知,癌症组织比癌旁组织具有更高的脂质含量。

The brightness of the synchrotron infrared light source is 100~1 000 times as high as the traditional globar light source.It has advantage on testing small sampleswith higher spatial resolution and signal to noise ratio.The bile of patients with benign and malignant tumors were compared by synchrotron micro-FTIR.The bile of patients with benign tumors contains much higher lipid than that of patient with malignant tumors,while the content of lecithin is on the opposite side.he composites and structure of bilirubin and proteinbetween the two samples are different.On the other hand,we find that the gallbladder carcinoma tissue contains much higher lipid than the adjacent tissue by means of the hierarchical cluster analysis.