Cu^(2+)参与的N-乙酰基-L-半胱氨酸修饰的CdTe量子点用于测定D-/L-青霉胺

N-Acetyl-L-Cysteine Capped CdTe Quantum Dots with Cu^(2+) Was Used for the Determination of D/L-Penicillamine

以N-乙酰基-L-半胱氨酸作为修饰剂,合成了高荧光产率的CdTe量子点。在pH为7.80的磷酸缓冲溶液中,Cu^(2+)可以显著猝灭量子点的荧光,有趣的是,在猝灭体系中加入手性青霉胺(PA)可使其荧光恢复。因此基于量子点的荧光变化,建立了快速检测D/L-青霉胺的方法。在最优的实验条件下,NALC-CdTe QDs-Cu^(2+)体系测定青霉胺的范围为2.0×10^(-7)～4.0×10^(-6) mol·L^(-1),检出限为5.8×10^(-8) mol·L^(-1).将该法成功用于实际样品中检测D/L-青霉胺,取得了满意的结果。

In this work,the CdTe quantum dots with high fluorescence yield were synthesized using N-acetyl-L-cysteine as a modifier.This study found that the fluorescence of CdTe quantum dots in pH 7.80 phosphate solution could be quenched by Cu2+,interestingly,the fluorescence can be recovered by adding chrial penicamines.Based on the fluorescence change,a simple method for detection of D/L-penicamines was proposed.Under the optimum conditions,the recovered fluorescence intensity was linearly proportional to the increasing penicamines concentration in the range of 2.0×10-7～4.0×10-6 mol·L-1,with the correlation coefficient was r2=0.998 0.This method has been demonstrated in real samples with satisfactory results.