快速全血凝集试验在中国不同人群HBsAg检测中的应用(英文)

Application of Rapid Whole-blood Agglutination Assay(RWBA) in Detection of HBsAg in Different Populations of China

为了评价本实验室建立的快速全血凝集法检测HBsAg的效果 ,检测了河北省不同人群 5 34份全血样品 ,得到了满意结果 .来自不同人群的 36 9份样品 (其中 6 2份来自乙肝患者 ,180份来自献血者 ,12 7份来自正常人群 ) ,经快速全血凝集法和国家认证的市售ELISA试剂盒平行检测后 ,总符合率为 96 .7% ,灵敏度为 90 .99% ,特异性为 98.7% .乙肝患者、献血者和正常人群中阳性率分别为 5 2 .6 % ,4 .3%和 8.9% .实验结果说明快速全血凝集法不仅简单快速、不需要特殊设备 ,而且具有高的敏感性和特异性 ,因此它可以广泛应用于基层卫生机构和临床HBsAg的检测 ,尤其适用于病人的床边诊断 .

In order to evaluate the efficacy of rapid whole-blood agglutination assay(RWBA) established in our laboratory,we tested 534 samples of whole-blood for HBsAg detection from different populatons in Hebei Province of China,and obtained satisfactory results.We detected HBsAg with whold-blood samples from different populations by RWBA,and compared with the results tested by commercial ELISA kit.The parallelled 369 samples from different populations were detected by both RWBA and commercial ELISA kit.Among them,there were 62 clinical patients with hepatitis B,180 cases of blood donors and 127 cases normal population.The total coincident index is 96.7%,the sensibility was 90.9%,and the specificity was 98.7%.The positive rate of population with clinical hepatitis,blood donors,and common population was 52.6%,4.3% and 8.9% respectively.The results indicated that RWBA was not only simpler and faster,need no special instruments,but also had higher sensiblity and specificity.It can be widely used in grass-roots health units and bedsides diagnosis for HBsAg detection.