人参皂苷Rh2及细胞因子诱导的杀伤细胞对人胃癌细胞株SGC-7901的体外增殖抑制作用

Proliferation inhibition effect of ginsenoside Rh2 and cytokine-induced killer cell on the SGC-7901 cells in vitro

目的研究人参皂苷Rh2、细胞因子诱导的杀伤(CIK)细胞及二者联合对胃癌细胞株SGC-7901的体外增殖抑制作用。方法本试验采用MTT法检测30、35、40及45μg/ml人参皂苷Rh2以及效靶比分别为5:1、10:1、20:1和40:1的CIK细胞,35μg/ml的人参皂苷Rh2联合效靶比20:1的CIK细胞,分别作用于SGC-7901细胞24 h和48 h的增殖抑制作用。结果不同剂量的人参皂苷Rh2作用于SGC-7901细胞,在相同作用时间内,抑制率随着剂量的增加而增加,差异有统计学意义(P<0.01);随给药时间的延长,除45μg/ml组外,其他各剂量组作用48 h的抑制率均较24 h有显著上升,差异有统计学意义(P<0.05或0.01)。不同效靶比的CIK细胞作用于SGC-7901细胞后,在相同作用时间内,抑制率随着效靶比的增加而增加,差异有统计学意义(P<0.01);相同效靶比条件下,抑制率随着作用时间的延长而增加,差异均具有统计学意义(P<0.01)。35μg/ml的人参皂苷Rh2联合20:1的CIK细胞对SGC-7901细胞的增殖抑制作用显著强于人参皂苷Rh2或CIK细胞单用,差异均具有统计学意义(P<0.05或0.01)。结论人参皂苷Rh2及CIK细胞在体外对胃癌细胞株SGC-7901均具有一定增殖抑制作用,且在一定的条件下二者联合应用具有协同效果,较单用具有更强的抑制SGC-7901细胞增殖的作用。

Objective To investigate the proliferation inhibition effect of ginsenoside Rh2 and cytokine-induced killer(CIK) cells and the both combination on human gastric cancer cell lines SGC-7901 in vitro.Methods The proliferation inhibition effect was measured by MTT assay.The inhibition rates of 30,35,40 and 45 ug/ml ginsenoside Rh2,effect target ratio 5:1,10:1,20:1 and 40:1 CIK cells,and 35 ug/ml ginsenoside Rh2 combined with effect target ratio 20:1 CIK cells effected on SGC-7901 cells were investigated.Results At the same effect time,the inhibition rates of ginsenoside Rh2 on SGC-7901 cells were increased followed the increase of dosage,and the differences were statistically significant(P<0.01).Excepted the 45 ug/ml group,48 h inhibition rate of other groups were increased significantly than 24 h,and the differences were statistically significant(P<0.05 or 0.01).At the same time,the inhibition rate of CIK cells were increased with the increase of effect target ratio,and the differences were statistically significant(P<0.01),and at the same effect target ratio condition,the inhibition rate increased followed the effect time,the differences were statistically significant(P<0.01).The inhibition effect of 35μg/ml Rh2 ginsenoside combined with effect target ratio 20:1 CIK cells on SGC-7901 cells was significantly stronger than that of ginsenoside Rh2 or CIK cells alone,the differences were statistically significant(P<0.05 or0.01).Conclusion Ginsenoside Rh2 and CIK cells both can inhibit SGC-7901 cell proliferation,and their combination may have a stronger or synergistic effect on inhibiting the proliferation of SGC-7901 cells.