湿疮1号对小鼠急性湿疹的影响及其可能机制

Effect and mechanism of Eczema prescription No.1 on the acute eczema in mice

目的研究湿疮1号对小鼠急性湿疹的抑制作用,探讨其可能的作用机制。方法采用2,4-二硝基氯苯(DNCB)诱导建立小鼠急性湿疹模型,随机分为5组,每组10只,分别为空白对照组(生理盐水)、阳性对照组(注射用氢化可的松琥珀酸钠)及湿疮1号不同剂量(低、中、高剂量)组,另选10只为正常对照组。观察小鼠耳部肿胀度,组织病理改变及血T辅助细胞1(Th1)和2(Th2)型细胞因子的表达情况。结果组织病理变化显示,空白对照组小鼠耳部表皮细胞间及细胞内水肿明显,血管扩张明显,淋巴细胞、血管周围炎性细胞浸润明显,药物干预后,与空白对照组比较,中药高、中剂量组和阳性对照组皮肤细胞间及细胞内水肿减轻,炎性细胞浸润较少。与阳性对照组比较,中药中、低剂量组的肿胀度明显增加,肿胀抑制率明显降低,差异有统计学意义(P<0.05);与正常对照组相比,造模后各组小鼠血清细胞因子IL-2、IFN-γ、IL-1β、TNF-α、IL-5均有明显升高,IL-4水平下降,差异有统计学意义(P<0.05)。干预后,与空白对照相比较,中药各剂量组及阳性对照组IL-2水平下降,IL-4水平升高,差异有统计学意义(P<0.05);中药高、中剂量组及阳性对照组IFN-γ、TNF-α水平下降,差异有统计学意义(P<0.05)。中药各剂量组间比较显示,中、低剂量组IL-2、IFN-γ、TNF-α水平明显高于高剂量组,差异有统计学意义(P<0.05)。结论湿疮1号对急性湿疹有明显的抑制作用,其作用机制可能与调节Th1/Th2细胞因子相关。

Objective To investigate the therapeutic effect and mechanism of Chinese medicine Eczema prescription No.1 on the acute eczema in mice. Methods Acute eczema model was established by applying 2, 4-dinitrochlorobenzene(DNCB) in mice. Then 50 mice were randomly divided into blank control group, positive control group, and three groups of different doses of Eczema prescription No.1 with 10 mice in each group. The blank control group was given normal saline, the positive control group was given hydrocortisone sodium succinate for injection, and additional 10 mice were selected as normal control group. The changes in mouse ear swelling and tissue pathology including the expressions of Th1 and Th2 cytokines were observed. Results According to the pathological changes, mice ear epidermis intracellular and intercellular edema, vascular dilation and lymphocytes and perivascular inflammatory cell infiltration in blank control group. After drug intervention, compared with the blank control group, the intercellular and intracellular edema were reduced and less inflammatory cell infiltration in moderate and high dose groups and positive control group(P<0.05). Compared with the positive control group, the swelling degree in moderate and low dose groups were significantly increased, and the inhibition rates were significantly lower, the differences were statistically significant(P<0.05). Compared with the normal control group, the levels of cytokines IL-2, IFN-γ, IL-1β, TNF-α, IL-5 in all models were significantly increased, and the IL-4 level was decreased, there were significant differences(P<0.05). After drug intervention, compared with blank control group, the level of IL-2 in all Chinese medicine groups and positive control group were decreased, while the level of IL-4 was increased with statistical significance(P<0.05). The levels of IFN-γ and TNF-α were decreased in high and moderate dose groups and positive control group(P<0.05). The levels of IL-2, IFN-γ and TNF-α in the moderate and low dose groups were significantly higher than those in high dose group(P<0.05). Conclusion Chinese medicine Eczema prescription No.1 is effective on acute eczema, which may be through the inhibition of Th1/Th2 type inflammation.