摘要
目的建立超高效液相色谱/串联质谱联用技术(UPLC-MS/MS法)测定大鼠血浆中肉桂酸的浓度,研究颈舒颗粒给药后肉桂酸的药代动力学。方法采用ACQUITY UPLC BEH C_(18)柱(1.7μm,2.1 mm×100 mm),甲醇-0.1%甲酸水梯度洗脱,流速为0.2 ml/min,进样量为5μl,柱温为35℃;Xevo TQ-S质谱在ESI+模式下采集数据,扫描方式为选择反应监测。结果肉桂酸在10~1 000 ng/ml浓度范围内呈现良好线性,批内、批间精密度均小于10%,稳定性良好。结论该方法准确、灵敏、专属,复方中药物的相互作用能改变肉桂酸在大鼠体内的吸收和代谢能力。
Objective We mainly intended to use the liquid chromatography electrospray ionisation tandem mass spectrometry to make the pharmacokinetics study on cinnamic acid contained in Jingshu granules.Methods With the type of chromatographic column was ACQUITY UPLC BEH C18(1.7μm 2.1 mm×100 mm),we used the methanol-0.1%formic acid for gradient elution system,with a flow rate of 0.2 ml/min,column temperature 35℃and injection volume 5μl.Xevo TQ-S mass spectrometry was used to collect data in ESI+mode by scanning for selective reaction monitoring.Results The cinnamic acid exhibited good linearity in the concentration range of 10~1 000 ng/ml.The intra-assay and inter-assay precision were less than 10%,and the stability was good.Conclusions The method was accurate,sensitive and exclusive.The interaction of drugs in the compound could change the absorption and metabolism of cinnamic acid in rats.
作者
陈瑞
许杉
律立红
徐智儒
刘莉
CHEN Rui;XU Shan;LYU Li-hong;XU Zhi-ru;LIU Li(Center for Pharmacological Evaluation and Research,China State Institute of Pharmaceutical Industry,Shanghai 200040,China;State Key Lab.of New Drug&Pharmaceutical Process,Shanghai Institute of Pharmaceutical Industry,China State Institute of Pharmaceutical Industry,Shanghai 200437,China;Shanghai Professional and Technical Service Center for Biological Material Druggability Evaluation,Shanghai 200437,China)
出处
《世界临床药物》
CAS
2019年第3期170-177,共8页
World Clinical Drug
基金
上海市科学技术委员会科研计划项目(15401903600)
上海市生物物质成药性评价专业技术服务平台(15DZ2291700)
上海市研发平台专项项目(18DZ2290900)
关键词
肉桂酸
颈舒颗粒
药代动力学
cinnamic acid
Jingshu granules
pharmacokinetics