摘要
目的 探讨烧伤继发急性肺损伤 (acutelunginjury ,ALI)患者肺毛细血管内皮细胞(PVEC)与中性粒细胞 (PMN)的粘附能力 ,观察C5a在PVEC PMN粘附行为中的作用程度及规律。 方法 采用微管吸吮技术 ,定量测定PVEC PMN之间粘附力值变化 ;测定髓过氧化酶 (MPO)活性 ,反映PVEC粘附PMN的数量。 结果 随着rh C5a浓度上升 ,PVEC粘附了更多的PMN ;粘附力值也逐渐增加 ,3 0 0 μg/Lrh C5a时差异有非常显著性意义 (P <0 .0 1) ;MPO也随之上升 ;工作浓度为 1∶10 4 的anti C5aRMcAb可缓解急性肺损伤 (ALI)血清所致的MPO增加 ,呈量效关系 , 结论 C5a、C5aR共同参与了PVEC PMN的粘附过程。而且急性肺损伤所致的粘附力值上升可被anti C5aRMcAb所抑制 ,提示C5a。
Objective To explore the postburn adhesion properties of polymorphonuclear leukocyte (PMN) onto pulmonary vascular endothelial cells (PVEC) in burn patients with acute lung injury (ALI), so as to determine the role of C5a on PVEC-PMN adhesion. Methods Microtubule sucking technique was employed to determine the PVEC-PMN adhesion.The myeloperoxidase (MPO) was also assayed to reflect the magnitude of PVEC-PMN adhesion. Results The magnitude of PVEC-PMN adhesion increased and the adhesion force increased along with an increase in rh-C5a concentration.Simultaneously, the MPO activity was increased, which could be inhibited by anti-C5aR McAb in a concentration 1∶104 . Conclusion Both C5a and C5aR participated in PVEC-PMN adhesion, which might be important in the pathogenesis of ALI.
出处
《中华烧伤杂志》
CAS
CSCD
2002年第6期358-361,共4页
Chinese Journal of Burns
基金
国家自然科学基金资助项目 (39970 330 )
