雷公藤多甙预处理亲缘皮修复烧伤创面的实验观察

An observation of repir of burn wound with consanguineous skin pretreated with Tripterygium wilfordii

目的 观察经雷公藤多甙预处理的亲缘皮移植烧伤创面后的效果。 方法 用新西兰大白兔 ,切取亲代厚中厚皮肤 ,分别用 2 0 0g/L雷公藤多甙、5 0 g/L地塞米松、9g/L等渗盐水浸泡 15～ 3 0min后 ,移植到子代小兔皮肤全层缺损处 ,同时设非亲缘皮对照组 ,观察创面移植物的存活时间及排斥情况。 结果 亲缘皮经雷公藤多甙预处理 ,移植到创面后排斥反应明显减轻 ,皮片存活时间明显延长 (P <0 .0 5 ) ,时间为 (4 3± 3 .5 )d。地塞米松组为 (3 0± 2 .5 )d。等渗盐水组和非亲缘皮组的皮片移植后排斥较快 ,平均为 (11± 1.6)d。 结论 亲缘皮的组织细胞有半同一性抗原 ,利用这一特点事先进行移植预处理 ,可以延长皮片排斥时间 。

Objective To explore new source of skin for burn wound coverage. Methods Split-thickness consanguineous skin was harvested from New Zealand white rabbit and was soaked in 200 g/L of multi-peptides of Tripterygium wilfordii, 50 g/L of dexamethasonel, on 9 g/L of normal saline solution for 15-30 mins, respectively. The consanguineous skin was thereafter grafted onto the whole layer skin defects in filial generation of rabbits with non-consanguineous skin as the control. The survival time and rejection of the grafted skin was observed. Results The rejection appeared evidently less intense and survived significantly longer (43±3.5 days)when the consanguineous skin was pretreated by Tripterygium wilfordii. However the grafted consanguineous skin survived for 30±2.5 days when it was pretreated by dexamethasone. The grafted skin was quickly rejected and survived only for 11±1.6 days when the skin was pretreated by normal saline or the skin was non-consanguineous. Conclusion Consanguineous skin possessed partial compatibility with the recipient due to similar antigen, which was beneficial to the its survival, especially after the skin was pretreated.