紫外分光光度法检测血清草甘膦中毒方法的建立和应用

Establishment and application of determination of glyphosate poisoning method by UV spectrophotometry

目的建立血清中草甘膦定性、定量测定的紫外分光光度法,为临床草甘膦中毒提供诊疗依据。方法取0.5mL血清加10%高氯酸甲醇溶液0.2mL充分振荡,以10 000r/min高速离心5min,取上清液进行亚硝基化反应,血清空白做基线,患者血清亚硝基化液50μL紫外扫描。结果血清中草甘膦最大吸收峰(243±l)nm,浓度在10.0~60.0μg/mL范围内呈线性,回归方程为Y=0.017 38 X+0.036 3(r=0.999 8),回收率为85.5%~102.4%,相对标准差(RSD)为3.50%~4.90%。日内、日间RSD分别为3.79%~5.10%和3.88%~4.55%,最低检出浓度5.0μg/mL。结论该方法操作简便、分析快速、结果准确。为临床诊断草甘膦中毒提供了一个简便准确的检测方法。

Objective To establish a qualitative and quantitative determination of glyphosate in serum using ultraviolet spectro-photometry(UV) to provide basis for clinical diagnosing and treating glyphosate poisoning .Methods The mixture of 0 .5 mL serum and 0 .2 mL 10% methanol solution of perchloric acid was shocked and centrifuged with 10 000 r/min for 5 min .A nitrosyla-tion reaction conducted on supernatant and 50 μL serum nitrosylation liquid was detected by UV scanning .Results The results of serum theophylline absorption maxima was(243 ± l) nm and the concentration of 10 .0-60 .0 μg/mL range linear regression equa-tion was Y=0 .0173 8X+0 .036 3(r= 0 .999 8) .The recovery rate was from 85 .5% to 102 .4% and the relative standard deviation (RSD) was from 3 .50% to 4 .90% .The intra-day and inter-day RSD were 3 .79% -5 .10% and 3 .88% -4 .55% .The minimum de-tectable concentration was 5μg/mL .Conclusion This method is simple ,rapid and accurate results for detecting glyphosate poison-ing .